Detailed information of TA system TA199706

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4518552..4519291
• Replicon: chromosome
• Accession: NZ_CP073310
• Organism: Enterobacter cloacae strain CZ862

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A9E6WPH7
• Locus tag: KDU74_RS22180
• Protein ID: WP_013087269.1
• Coordinates: 4518806..4519291 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A9E6WPN8
• Locus tag: KDU74_RS22175
• Protein ID: WP_007869691.1
• Coordinates: 4518552..4518818 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KDU74_RS22150 (KDU74_22150)	4513746..4514918	+	1173	WP_023328878.1	transporter substrate-binding domain-containing protein	-
KDU74_RS22155 (KDU74_22155)	4514968..4515543	+	576	WP_221929658.1	ANTAR domain-containing protein	-
KDU74_RS22160 (KDU74_22160)	4515594..4517327	+	1734	WP_032665819.1	nitrilase-related carbon-nitrogen hydrolase	-
KDU74_RS22165 (KDU74_22165)	4517449..4517598	-	150	WP_211944391.1	transposase domain-containing protein	-
KDU74_RS22170 (KDU74_22170)	4517681..4518378	+	698	WP_249166270.1	IS1 family transposase	-
KDU74_RS22175 (KDU74_22175)	4518552..4518818	+	267	WP_007869691.1	DUF1778 domain-containing protein	Antitoxin
KDU74_RS22180 (KDU74_22180)	4518806..4519291	+	486	WP_013087269.1	GNAT family N-acetyltransferase	Toxin
KDU74_RS22185 (KDU74_22185)	4519487..4520890	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
KDU74_RS22190 (KDU74_22190)	4520919..4521551	-	633	WP_001567369.1	hypothetical protein	-
KDU74_RS22195 (KDU74_22195)	4521626..4521997	+	372	Protein_4350	IS5/IS1182 family transposase	-
KDU74_RS22200 (KDU74_22200)	4522029..4522997	+	969	WP_013815099.1	IS5-like element IS903B family transposase	-
KDU74_RS22205 (KDU74_22205)	4523158..4523973	+	816	WP_012695470.1	aminoglycoside O-phosphotransferase APH(3')-Ia	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	aph(3')-Ia	-	4496277..4544800	48523
	-	inside	IScluster/Tn	aph(3')-Ia	-	4517681..4529233	11552

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17736.45 Da
• Isoelectric Point: 9.5181

>T199706 WP_013087269.1 NZ_CP073310:4518806-4519291 [Enterobacter cloacae]
VGRVTAPEPLSSSHQLAEFVSGEAVLDDWLKQRGLKNQALGAARTFVVCKRDTKQVVGFYSLATGSVNHVEAMGSLRRNM
PDPIPVIILARLAVDVSYRGQGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYLHHGFKASQTQERTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T199706 NZ_CP097528:c1778153-1777875 [Pseudomonas putida]
ATGATTCGAAGCTTTAGCTGTGCCGACACGGAAGCGCTCTTCACAACGGGAAAGACACGGCGCGGGTCAGATATCAAGTC
AGTCGCGGAGCGCAAGCTGGCCATGCTCGATGCCGCAACAGAGTTGCGAGACCTTAGGTCGCCACCTGGCAATCGCCTCG
AGTCACTGAGCGGCAACCGCGCCGACCAGCACAGTATCCGTGTAAATGACCAATGGCGGTTGTGCTTCACCTGGACAGAG
CACGGGCCAGTTAACGTTGAAATTGTCGATTACCACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10080.46 Da
• Isoelectric Point: 4.3837

>AT199706 WP_007869691.1 NZ_CP073310:4518552-4518818 [Enterobacter cloacae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVSDEAAIDKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT199706 NZ_CP097528:c1777866-1777567 [Pseudomonas putida]
ATGCTCAAGAACGGTATGCGTCCAATCCATCCGGGTGAAATCCTGCGGGAGGAGTTCCAGAAGGAAATGGGCTTCAGCGC
CGCGGCTCTGGCCAGGGCGTTGGGTGTAGCAACGCCAACCGTCAACAACATCCTGCGCGAACGGGGCGGTGTTTCCGCCG
ACATGGCACTGCGCCTGTCCATCTGCCTGGACACCACCCCTGAGTTCTGGCTCAACCTGCAGACGGCCTTCGATCTGCGC
ACAGCCGAACAGCAACACGGCGATGAGATCATCGGGTCGGTCCAGCGTCTGGTCGCTTGA