Detailed information of TA system TA199703

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3512616..3513132
• Replicon: chromosome
• Accession: NZ_CP073310
• Organism: Enterobacter cloacae strain CZ862

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KDU74_RS17090
• Protein ID: WP_178328142.1
• Coordinates: 3512616..3512900 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V3EXX3
• Locus tag: KDU74_RS17095
• Protein ID: WP_008501400.1
• Coordinates: 3512890..3513132 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KDU74_RS17075 (KDU74_17075)	3507870..3509513	+	1644	WP_178328143.1	alpha,alpha-phosphotrehalase	-
KDU74_RS17080 (KDU74_17080)	3509887..3512025	+	2139	WP_013095357.1	anaerobic ribonucleoside-triphosphate reductase	-
KDU74_RS17085 (KDU74_17085)	3512148..3512612	+	465	WP_058683233.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
KDU74_RS17090 (KDU74_17090)	3512616..3512900	-	285	WP_178328142.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KDU74_RS17095 (KDU74_17095)	3512890..3513132	-	243	WP_008501400.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
KDU74_RS17100 (KDU74_17100)	3513210..3515120	-	1911	WP_178328141.1	PRD domain-containing protein	-
KDU74_RS17105 (KDU74_17105)	3515140..3516276	-	1137	WP_178328140.1	lactonase family protein	-
KDU74_RS17110 (KDU74_17110)	3516391..3517130	-	740	Protein_3350	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10944.80 Da
• Isoelectric Point: 10.4610

>T199703 WP_178328142.1 NZ_CP073310:c3512900-3512616 [Enterobacter cloacae]
MTYELEFDPRALKEWSKLGETVKKQFRKKLAGVLVNPRIESARLHNLPDCYKIKLRSQGYRLVYQVQDNVVTVVVVAIGK
REKSAVYHDANKRL

Nucleotide

• Length: 285 bp

>T199703 NZ_CP097528:332741-333217 [Pseudomonas putida]
TTGACGGGCATCAATCCATTGGCGATCGCCAGGTCACCGTACATCGCGCATCCAGATATCTGGTTGACAGATATCTGGAT
CGGAGATATTTTCCACCCTATGTTGGTGCGCCGGGACAACACAATGCGGGTCATAACGAAAGCGGCGGTTACCAAGGCGA
TTGAAGTCCACGGCCAGTGGAAAGCGCCCCTAAGCCTGTGGCTGACGACCTTCGACAGGGCGACGCTGCGGTTCGAATCT
TTTGAACAGTTGAGGCAGACGTGGGCCACTGTGTCTGGCTGGAATGTTGATCGCATCCCCCACTCAAAACTCAGGCCCGC
CAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGC
GAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGACAAATGGTGCAGAAGCGATATCAGGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8972.37 Da
• Isoelectric Point: 5.0651

>AT199703 WP_008501400.1 NZ_CP073310:c3513132-3512890 [Enterobacter cloacae]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTISDSEDALIQTVRERLANPQKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT199703 NZ_CP097528:333259-333732 [Pseudomonas putida]
ATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCTCGATGGCCTGAACAC
TCAGATGGCAACCATGGCCCAGCTTCTAGCCGCCTGCCATGACCCCATCAAGGACGATGCCGAGCTTGAGGCACGCATGG
CCTTGATCGACGAGCTCTACTCCCATGCGGACAGCGAAGGCCACGCTGCGGCACGCTTTGCCGAGATGGTAGCGGACCGC
GTCTACGAGTATGAGAGTGAGTCTGTATTGGTGCCGTTCGCGAGCCAGGCCGAAGCGTTGGCCTTTCTGCTGTCGGATAG
GGGCGTGAAGCAGAAGGACCTGTCGGCGATAGCGACCCAGAGCGCGGTATCCGAGATTCTGAACAACAAGCGGAAGATGA
CGGTTGCCCAGATAAAGGGCTTTGCTGAGTTTTTCAGCGTACCGGTTGAGTTCTTCATGCATGGGGTGGTTTGA

Structures

Antitoxin

• AlphaFold DB: V3EXX3