Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3355360..3355976 Replicon chromosome
Accession NZ_CP073310
Organism Enterobacter cloacae strain CZ862

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag KDU74_RS16325 Protein ID WP_080292722.1
Coordinates 3355605..3355976 (+) Length 124 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag KDU74_RS16320 Protein ID WP_178328918.1
Coordinates 3355360..3355602 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KDU74_RS16295 (KDU74_16295) 3350605..3351486 + 882 WP_013099364.1 virulence factor BrkB family protein -
KDU74_RS16300 (KDU74_16300) 3351483..3351920 + 438 WP_013099363.1 D-aminoacyl-tRNA deacylase -
KDU74_RS16305 (KDU74_16305) 3351965..3352906 + 942 WP_013099362.1 fatty acid biosynthesis protein FabY -
KDU74_RS16310 (KDU74_16310) 3352919..3353836 - 918 WP_178328392.1 alpha/beta hydrolase -
KDU74_RS16315 (KDU74_16315) 3353931..3355151 + 1221 WP_065945178.1 ISL3-like element ISCfr12 family transposase -
KDU74_RS16320 (KDU74_16320) 3355360..3355602 + 243 WP_178328918.1 ribbon-helix-helix protein, CopG family Antitoxin
KDU74_RS16325 (KDU74_16325) 3355605..3355976 + 372 WP_080292722.1 type II toxin-antitoxin system VapC family toxin Toxin
KDU74_RS16330 (KDU74_16330) 3356016..3356945 - 930 WP_029882351.1 formate dehydrogenase accessory protein FdhE -
KDU74_RS16335 (KDU74_16335) 3356942..3357577 - 636 WP_013099357.1 formate dehydrogenase cytochrome b556 subunit -
KDU74_RS16340 (KDU74_16340) 3357574..3358476 - 903 WP_014833793.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3353931..3355151 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-115)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-49)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13625.69 Da        Isoelectric Point: 6.6407

>T199702 WP_080292722.1 NZ_CP073310:3355605-3355976 [Enterobacter cloacae]
VNNMAVFDTNILIDLFNNRVEAADAIDHAASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFSGIDEVVTPYQL

Download         Length: 372 bp

>T199702 NZ_CP097527:c4750764-4750468 [Pseudomonas putida]
ATGGAAAAGAGAATGCCTCATTGCCCGCTGGAGCGGGTAAAAGCCCTGGCTGCGGCAAGGCGAATCCGCCCTACGGGTGC
TGCATTGAAAGGCGCCAAGGCGCTGGGAATGGATTTTCCGGGCATGCTTGAGGTGATCACCAGCTTGAAGCGCACTGATT
TCTACAAGAGCATGACCAGCCATATTGACCACCGGGTTTGGCAGGATGTGTATCGCCCGCTTACAGCAATTGGGTACGTT
TATTTGAAATTGTCCGTGGTAGACGACGTGCTCATCGTGTCTTTCAAGGAGTTGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9379.76 Da        Isoelectric Point: 4.5386

>AT199702 WP_178328918.1 NZ_CP073310:3355360-3355602 [Enterobacter cloacae]
MSVLAELDMGRILIDLPDDVIQRLDNLKKLRNQPRAELLREAIELYLDQQSSSVIRAALGLWGDKKEDGLEYERKLREEW

Download         Length: 243 bp

>AT199702 NZ_CP097527:c4750465-4750064 [Pseudomonas putida]
ATGAGATGTCCAATATGCGGCGGCTCGGAGCTTGCACCTGACATTCAGGGCATGCCCTATAGCTACAAAGGCGAGATGAC
AGTGATTCCTGAGGTAAGTGGCGATTACTGCTCCGCATGTGGTGAATGTGTACTGAGTCATGATGAAGCCATGCGCGTCA
GTCACTTGATGACGGCATTCGAGCGCCAGGTCAACGCAAATGTTGTGGATCCTTCCTTTATTGCCTCCATACGCAGAAAG
TTCGACCTCGACCAGCGTGAAGCGGGGGAAATCTTCGGTGGTGGGGTCAATGCGTTCTCCCGTTATGAAAACGGCAAGAC
CACCCCGCCAGTGGCGTTGGTGAAACTGCTCAAGCTGCTGGATCGCCATCCAGAACTCTTTGAGGAAGTGCGTACTGCCT
GA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References