Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1730366..1730986 Replicon chromosome
Accession NZ_CP073310
Organism Enterobacter cloacae strain CZ862

Toxin (Protein)


Gene name Hha Uniprot ID A0A5E1A1U8
Locus tag KDU74_RS08515 Protein ID WP_013095889.1
Coordinates 1730768..1730986 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag KDU74_RS08510 Protein ID WP_008499288.1
Coordinates 1730366..1730740 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KDU74_RS08500 (KDU74_08500) 1725491..1726684 + 1194 WP_178328549.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KDU74_RS08505 (KDU74_08505) 1726707..1729853 + 3147 WP_178328548.1 multidrug efflux RND transporter permease subunit AcrB -
KDU74_RS08510 (KDU74_08510) 1730366..1730740 + 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
KDU74_RS08515 (KDU74_08515) 1730768..1730986 + 219 WP_013095889.1 HHA domain-containing protein Toxin
KDU74_RS08520 (KDU74_08520) 1731196..1731747 + 552 WP_013095888.1 maltose O-acetyltransferase -
KDU74_RS08525 (KDU74_08525) 1731863..1732330 + 468 WP_013095887.1 YlaC family protein -
KDU74_RS08530 (KDU74_08530) 1732302..1733762 - 1461 WP_150057013.1 PLP-dependent aminotransferase family protein -
KDU74_RS08535 (KDU74_08535) 1733864..1734574 + 711 WP_023620096.1 GNAT family protein -
KDU74_RS08540 (KDU74_08540) 1734571..1734711 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
KDU74_RS08545 (KDU74_08545) 1734714..1734974 - 261 WP_006176933.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8625.98 Da        Isoelectric Point: 8.9008

>T199700 WP_013095889.1 NZ_CP073310:1730768-1730986 [Enterobacter cloacae]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELTVFYSAADHRLAELTMNKLYDKIPPSVWKFVR

Download         Length: 219 bp

>T199700 NZ_CP097527:c4415659-4415411 [Pseudomonas putida]
ATGTCTAAGCAAGAAAAGCTGCTCGCGAAGCTGCTGAACAAGCAAGCAGGATTCACTTGGCCTGAGCTGGTCACGCTGCT
GGGTGGGCTTGGATACCGCCATATCGAGGGTGATGGCAGCCGAGTGAAGTTCGACAATGGCCACCCGCAGGCAATGATCA
ACTTGCACAAGCCGCACCCCGGCAATGAGCTGAAGGCCTACGTTAAACGCCAGGTAATAGAGCACCTGAAAGCAGGAGGA
TTGATCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT199700 WP_008499288.1 NZ_CP073310:1730366-1730740 [Enterobacter cloacae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT199700 NZ_CP097527:c4415411-4415070 [Pseudomonas putida]
ATGAGCACCATGTTGCAGTACCGAGGCTACTACGGCTCCATTGAGGCCAGCCCCGAGGACAATTGCCTATTCGGCAAGCT
TCAGTTCATCCGCGCCCTTGTCAGCTATGAGGGCGAGACGGTTGCTGAACTGACCCAGGCGTTCCGCGATGCAGTGGACG
ACTATCTCGACACCTGCGCATCACTCGGGCAGAACCCGGAAATTCCGTGCAAGGGGTCGTTCAATGTGAGGGTAGGGCAC
GACCTGCACCTAGCGGCCAGCGTGGCAGCCACCAAGCAAAGTATTTCCTTGAATGATCTGACACGCAAAGCGCTTAGTGA
GTACCTGGGTCATCACGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1A1U8

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References