Detailed information of TA system TA199690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 5834..6414
• Replicon: plasmid pB
• Accession: NZ_CP073304
• Organism: Klebsiella pneumoniae strain SW1780

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: KCX81_RS27950
• Protein ID: WP_071177730.1
• Coordinates: 6100..6414 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: KCX81_RS27945
• Protein ID: WP_000093040.1
• Coordinates: 5834..6112 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCX81_RS27915 (KCX81_27915)	1894..2043	-	150	WP_032440454.1	colicin release lysis protein	-
KCX81_RS27920 (KCX81_27920)	2128..2385	-	258	WP_032440455.1	colicin E3-like toxin immunity protein	-
KCX81_RS27925 (KCX81_27925)	2395..4080	-	1686	WP_032440457.1	colicin-like bacteriocin tRNase domain-containing protein	-
KCX81_RS27930 (KCX81_27930)	4407..4652	+	246	WP_032440458.1	hypothetical protein	-
KCX81_RS27935 (KCX81_27935)	4926..5297	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
KCX81_RS28225	5294..5659	+	366	WP_072354022.1	TonB family protein	-
KCX81_RS27945 (KCX81_27945)	5834..6112	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
KCX81_RS27950 (KCX81_27950)	6100..6414	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KCX81_RS27955 (KCX81_27955)	6578..7006	+	429	WP_001140599.1	hypothetical protein	-
KCX81_RS27960 (KCX81_27960)	7032..7211	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
KCX81_RS27965 (KCX81_27965)	7238..7768	-	531	WP_071177729.1	hypothetical protein	-
KCX81_RS27970 (KCX81_27970)	7775..8506	-	732	WP_071177728.1	MobC family replication-relaxation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10060	10060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T199690 WP_071177730.1 NZ_CP073304:6100-6414 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T199690 NZ_CP097519:800645-801007 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT199690 WP_000093040.1 NZ_CP073304:5834-6112 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT199690 NZ_CP097519:800478-800648 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTCCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCAA
AGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1