Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 69085..69728 Replicon plasmid pSW1780-KPC
Accession NZ_CP073303
Organism Klebsiella pneumoniae strain SW1780

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag KCX81_RS27525 Protein ID WP_001044770.1
Coordinates 69085..69501 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag KCX81_RS27530 Protein ID WP_001261282.1
Coordinates 69498..69728 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KCX81_RS27505 (65188) 65188..65460 - 273 Protein_88 transposase -
KCX81_RS27515 (66442) 66442..67464 - 1023 WP_000361404.1 helicase UvrD -
KCX81_RS27520 (67449) 67449..69011 - 1563 WP_004206609.1 AAA family ATPase -
KCX81_RS27525 (69085) 69085..69501 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
KCX81_RS27530 (69498) 69498..69728 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KCX81_RS27535 (69685) 69685..70146 + 462 WP_014343465.1 hypothetical protein -
KCX81_RS27540 (70307) 70307..71251 + 945 WP_011977810.1 hypothetical protein -
KCX81_RS27545 (71288) 71288..71680 + 393 WP_011977811.1 hypothetical protein -
KCX81_RS27550 (71738) 71738..72259 + 522 WP_013214008.1 hypothetical protein -
KCX81_RS27555 (72305) 72305..72508 + 204 WP_011977813.1 hypothetical protein -
KCX81_RS27560 (72538) 72538..73542 + 1005 WP_011977814.1 hypothetical protein -
KCX81_RS27565 (73726) 73726..74505 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-65 / fosA3 / rmtB / blaTEM-1B / blaKPC-2 / catA2 - 1..125619 125619


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T199687 WP_001044770.1 NZ_CP073303:c69501-69085 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T199687 NZ_CP097514:1071293-1071472 [Staphylococcus epidermidis]
ATGGCGAACAAAAAAGATTCCAAATTAAATTATCATGAAGAAGAAAATGCTATGGTTACCGACTTAGATGATTTAAAAGA
ATTAGGAAAAGAAATGGAACAGATTTCACAGGAAAATGATGAAGAAAAATTAAATCAATCTCATGATAACGAAGTTCGTT
CAGATTTAAAAAAACAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT199687 WP_001261282.1 NZ_CP073303:c69728-69498 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT199687 NZ_CP097514:1070670-1071269 [Staphylococcus epidermidis]
ATGGCAATGAATTTTAAAGTATTTGATAATAGTGAAAAAGTAGCAGAATATACTGCAGATATTCTACGTAAGCAGTTTAA
TAATAATCCAACTACTATTGCAGGATTTCATTTGAGCAAAGAACATGCACCAGTGTTTGATGAACTTAAGAAAAATGTAG
AAAATCATACAGTTGATTTCAGTCAAATAAATATTTTAGATTATGATGATAATCATTCTTATTATGAGGCTTTGGGTGTG
CCAACAGGCCAAATTTATTCCATTTCTTACGAAAATGATGCAATTGACTTTATCTCAGATAAGATTAAGACTAAAGAAAA
TAAAGGCAAACTGACAATGCAAGTCCTTACGATTGATGAAAATGGTAAACTTGACATTAGCGTTCGTCAAGGTTTGATGG
AAGCTAGAGAAATTTTCTTAGTTATTACTGGTGCTAATAAACGCGATATGGTAGAAAAACTTTACCGTGAAAATGGTAAG
ACTAGTTTTGAACCATCGGATTTAAAAGCACACCGTATGGTCAATGTTATCTTAGATAAAGAAGCGGCTGCAGGCTTACC
TGAAGACGTAAAAGAGTACTTCACTGCACGTTTTGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References