Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5414813..5415438 Replicon chromosome
Accession NZ_CP073302
Organism Klebsiella pneumoniae strain SW1780

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KCX81_RS26705 Protein ID WP_002882817.1
Coordinates 5414813..5415196 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KCX81_RS26710 Protein ID WP_004150355.1
Coordinates 5415196..5415438 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KCX81_RS26690 (KCX81_26690) 5412179..5413081 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KCX81_RS26695 (KCX81_26695) 5413078..5413713 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KCX81_RS26700 (KCX81_26700) 5413710..5414639 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KCX81_RS26705 (KCX81_26705) 5414813..5415196 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KCX81_RS26710 (KCX81_26710) 5415196..5415438 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KCX81_RS26715 (KCX81_26715) 5415643..5416560 + 918 WP_002882812.1 alpha/beta hydrolase -
KCX81_RS26720 (KCX81_26720) 5416574..5417515 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KCX81_RS26725 (KCX81_26725) 5417560..5417997 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KCX81_RS26730 (KCX81_26730) 5417994..5418854 - 861 WP_002882807.1 virulence factor BrkB family protein -
KCX81_RS26735 (KCX81_26735) 5418848..5419447 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T199683 WP_002882817.1 NZ_CP073302:c5415196-5414813 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T199683 NZ_CP097512:1071299-1071478 [Staphylococcus epidermidis]
ATGGCGAACAAAAAAGATTCCAAATTAAATTATCATGAAGAAGAAAATGCTATGGTTACCGACTTAGATGATTTAAAAGA
ATTAGGAAAAGAAATGGAACAGATTTCACAGGAAAATGATGAAGAAAAATTAAATCAATCTCATGATAACGAAGTTCGTT
CAGATTTAAAAAAACAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT199683 WP_004150355.1 NZ_CP073302:c5415438-5415196 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT199683 NZ_CP097512:1070676-1071275 [Staphylococcus epidermidis]
ATGGCAATGAATTTTAAAGTATTTGATAATAGTGAAAAAGTAGCAGAATATACTGCAGATATTCTACGTAAGCAGTTTAA
TAATAATCCAACTACTATTGCAGGATTTCATTTGAGCAAAGAACATGCACCAGTGTTTGATGAACTTAAGAAAAATGTAG
AAAATCATACAGTTGATTTCAGTCAAATAAATATTTTAGATTATGATGATAATCATTCTTATTATGAGGCTTTGGGTGTG
CCAACAGGCCAAATTTATTCCATTTCTTACGAAAATGATGCAATTGACTTTATCTCAGATAAGATTAAGACTAAAGAAAA
TAAAGGCAAACTGACAATGCAAGTCCTTACGATTGATGAAAATGGTAAACTTGACATTAGCGTTCGTCAAGGTTTGATGG
AAGCTAGAGAAATTTTCTTAGTTATTACTGGTGCTAATAAACGCGATATGGTAGAAAAACTTTACCGTGAAAATGGTAAG
ACTAGTTTTGAACCATCGGATTTAAAAGCACACCGTATGGTCAATGTTATCTTAGATAAAGAAGCGGCTGCAGGCTTACC
TGAAGACGTAAAAGAGTACTTCACTGCACGTTTTGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References