Detailed information of TA system TA199669

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 877832..878486
• Replicon: chromosome
• Accession: NZ_CP073302
• Organism: Klebsiella pneumoniae strain SW1780

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: R4YHX2
• Locus tag: KCX81_RS04420
• Protein ID: WP_004144731.1
• Coordinates: 878079..878486 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W8UQ37
• Locus tag: KCX81_RS04415
• Protein ID: WP_002916312.1
• Coordinates: 877832..878098 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCX81_RS04390 (KCX81_04390)	872988..874421	-	1434	WP_002916322.1	6-phospho-beta-glucosidase BglA	-
KCX81_RS04395 (KCX81_04395)	874540..875268	-	729	WP_002916321.1	MurR/RpiR family transcriptional regulator	-
KCX81_RS04400 (KCX81_04400)	875318..875629	+	312	WP_002916319.1	N(4)-acetylcytidine aminohydrolase	-
KCX81_RS04405 (KCX81_04405)	875793..876452	+	660	WP_002916317.1	hemolysin III family protein	-
KCX81_RS04410 (KCX81_04410)	876603..877586	-	984	WP_002916313.1	tRNA-modifying protein YgfZ	-
KCX81_RS04415 (KCX81_04415)	877832..878098	+	267	WP_002916312.1	FAD assembly factor SdhE	Antitoxin
KCX81_RS04420 (KCX81_04420)	878079..878486	+	408	WP_004144731.1	protein YgfX	Toxin
KCX81_RS04425 (KCX81_04425)	878493..879014	-	522	WP_002916308.1	flavodoxin FldB	-
KCX81_RS04430 (KCX81_04430)	879115..880011	+	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
KCX81_RS04435 (KCX81_04435)	880034..880747	+	714	WP_002916301.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
KCX81_RS04440 (KCX81_04440)	880753..882486	+	1734	WP_004151783.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15936.69 Da
• Isoelectric Point: 11.4778

>T199669 WP_004144731.1 NZ_CP073302:878079-878486 [Klebsiella pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNAE
WEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Nucleotide

• Length: 408 bp

>T199669 NZ_CP097467:c1321934-1321188 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCACTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAAAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10480.98 Da
• Isoelectric Point: 5.1116

>AT199669 WP_002916312.1 NZ_CP073302:877832-878098 [Klebsiella pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT199669 NZ_CP097467:c1321187-1321017 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: A0A378E4P6

Antitoxin

• AlphaFold DB: A0A0H3GY41