Detailed information of TA system TA199666

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 330427..331013
• Replicon: chromosome
• Accession: NZ_CP073302
• Organism: Klebsiella pneumoniae strain SW1780

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: KCX81_RS01535
• Protein ID: WP_002920800.1
• Coordinates: 330645..331013 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A0H3GZM4
• Locus tag: KCX81_RS01530
• Protein ID: WP_002920802.1
• Coordinates: 330427..330648 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCX81_RS01510 (KCX81_01510)	326584..327510	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
KCX81_RS01515 (KCX81_01515)	327507..328784	+	1278	WP_002920806.1	branched chain amino acid ABC transporter permease LivM	-
KCX81_RS01520 (KCX81_01520)	328781..329548	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
KCX81_RS01525 (KCX81_01525)	329550..330263	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
KCX81_RS01530 (KCX81_01530)	330427..330648	+	222	WP_002920802.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KCX81_RS01535 (KCX81_01535)	330645..331013	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KCX81_RS01540 (KCX81_01540)	331286..332602	+	1317	WP_002920796.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
KCX81_RS01545 (KCX81_01545)	332709..333596	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
KCX81_RS01550 (KCX81_01550)	333593..334438	+	846	WP_002920789.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
KCX81_RS01555 (KCX81_01555)	334440..335510	+	1071	WP_002920787.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	325413..336247	10834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-49)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T199666 WP_002920800.1 NZ_CP073302:330645-331013 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T199666 NZ_CP097464:480719-481042 [Leptospira borgpetersenii]
ATGGTGATTTCTCAATACGAAGTATATTTGATAAATTTGGATCCAACGGTCGGACATGAGATTAAAAAATCAAGACCTTG
CGTAATAATCTCACCTAATGAAATGAATAAAACTATCGGAACAATTATTATTACACCAATGACTACAAAATCTCGATCAT
ATCCAACAAGAGTCGAATTAACATTTCAAGGAAAGAAAGGCTGGATTGTGTTAGATCAAATTCGAACTGTAGATAAAATA
CGTCTGATAAAAAAGTTAGGAAAAATAGATCCCAAAACAGTTAATAAAATGAAACTGATTATAAAGGAAATGTTGGTTGA
TTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8181.18 Da
• Isoelectric Point: 4.7769

>AT199666 WP_002920802.1 NZ_CP073302:330427-330648 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT199666 NZ_CP097464:480477-480725 [Leptospira borgpetersenii]
ATGAAGGCTTTAGTTGTAAAAATAGGTAATTCAAAAGGAATTCGGATTCCCAAAGCGGTTTTAGAAGAATGCCATATTGA
AGAAGAAGTAGATTTACTAATCGATAACAATAAATTAATTATTGTGCCTCTAAAATCGAAACCAAGAGAAGGGTGGGAAA
AACAATTTAAATCAATGTCATCTAATAAAGATGATAAATTGCTTATTCCTGATTCAATAGATTTATCGGATAAGGATTGG
GAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A0H3GZM4