Detailed information of TA system TA199665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 154940..155610
• Replicon: plasmid pVir_030925
• Accession: NZ_CP073286
• Organism: Klebsiella pneumoniae subsp. ozaenae strain WCHKP030925

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: CLQ62_RS27180
• Protein ID: WP_004213072.1
• Coordinates: 154940..155383 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: CLQ62_RS27185
• Protein ID: WP_004213073.1
• Coordinates: 155380..155610 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLQ62_RS27145 (CLQ62_27125)	150350..150625	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
CLQ62_RS27150 (CLQ62_27130)	150688..151179	+	492	WP_011251327.1	DM13 domain-containing protein	-
CLQ62_RS27155 (CLQ62_27135)	151228..152148	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
CLQ62_RS27160 (CLQ62_27140)	152239..152642	+	404	Protein_166	GAF domain-containing protein	-
CLQ62_RS27165 (CLQ62_27145)	153160..153796	-	637	Protein_167	mucoid phenotype regulator RmpA2	-
CLQ62_RS27170 (CLQ62_27150)	154213..154517	+	305	Protein_168	transposase	-
CLQ62_RS27750	154540..154791	-	252	WP_186987481.1	hypothetical protein	-
CLQ62_RS27180 (CLQ62_27160)	154940..155383	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
CLQ62_RS27185 (CLQ62_27165)	155380..155610	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CLQ62_RS27190 (CLQ62_27170)	156218..157351	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
CLQ62_RS27195 (CLQ62_27175)	157367..157660	+	294	WP_004213076.1	hypothetical protein	-
CLQ62_RS27200 (CLQ62_27180)	157650..157856	-	207	WP_004213077.1	hypothetical protein	-
CLQ62_RS27205 (CLQ62_27185)	158208..158498	+	291	WP_004213078.1	hypothetical protein	-
CLQ62_RS27210 (CLQ62_27190)	158488..159387	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..228949	228949

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T199665 WP_004213072.1 NZ_CP073286:c155383-154940 [Klebsiella pneumoniae subsp. ozaenae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T199665 NZ_CP097464:480477-480725 [Leptospira borgpetersenii]
ATGAAGGCTTTAGTTGTAAAAATAGGTAATTCAAAAGGAATTCGGATTCCCAAAGCGGTTTTAGAAGAATGCCATATTGA
AGAAGAAGTAGATTTACTAATCGATAACAATAAATTAATTATTGTGCCTCTAAAATCGAAACCAAGAGAAGGGTGGGAAA
AACAATTTAAATCAATGTCATCTAATAAAGATGATAAATTGCTTATTCCTGATTCAATAGATTTATCGGATAAGGATTGG
GAATGGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT199665 WP_004213073.1 NZ_CP073286:c155610-155380 [Klebsiella pneumoniae subsp. ozaenae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT199665 NZ_CP097464:480719-481042 [Leptospira borgpetersenii]
ATGGTGATTTCTCAATACGAAGTATATTTGATAAATTTGGATCCAACGGTCGGACATGAGATTAAAAAATCAAGACCTTG
CGTAATAATCTCACCTAATGAAATGAATAAAACTATCGGAACAATTATTATTACACCAATGACTACAAAATCTCGATCAT
ATCCAACAAGAGTCGAATTAACATTTCAAGGAAAGAAAGGCTGGATTGTGTTAGATCAAATTCGAACTGTAGATAAAATA
CGTCTGATAAAAAAGTTAGGAAAAATAGATCCCAAAACAGTTAATAAAATGAAACTGATTATAAAGGAAATGTTGGTTGA
TTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620