Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5393153..5393778 Replicon chromosome
Accession NZ_CP073285
Organism Klebsiella pneumoniae subsp. ozaenae strain WCHKP030925

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag CLQ62_RS25990 Protein ID WP_012737091.1
Coordinates 5393153..5393536 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag CLQ62_RS25995 Protein ID WP_004150355.1
Coordinates 5393536..5393778 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLQ62_RS25975 (5390519) 5390519..5391421 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
CLQ62_RS25980 (5391418) 5391418..5392053 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
CLQ62_RS25985 (5392050) 5392050..5392979 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
CLQ62_RS25990 (5393153) 5393153..5393536 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
CLQ62_RS25995 (5393536) 5393536..5393778 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
CLQ62_RS26000 (5393983) 5393983..5394900 + 918 WP_012737090.1 alpha/beta hydrolase -
CLQ62_RS26005 (5394914) 5394914..5395855 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
CLQ62_RS26010 (5395900) 5395900..5396337 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
CLQ62_RS26015 (5396334) 5396334..5397194 - 861 WP_004146232.1 virulence factor BrkB family protein -
CLQ62_RS26020 (5397188) 5397188..5397787 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T199662 WP_012737091.1 NZ_CP073285:c5393536-5393153 [Klebsiella pneumoniae subsp. ozaenae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T199662 NZ_CP097431:7594-7746 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT199662 WP_004150355.1 NZ_CP073285:c5393778-5393536 [Klebsiella pneumoniae subsp. ozaenae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT199662 NZ_CP097431:c7539-7482 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References