Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4755981..4756791 Replicon chromosome
Accession NZ_CP073285
Organism Klebsiella pneumoniae subsp. ozaenae strain WCHKP030925

Toxin (Protein)


Gene name KacT Uniprot ID A0A332NV98
Locus tag CLQ62_RS23010 Protein ID WP_014908042.1
Coordinates 4755981..4756514 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag CLQ62_RS23015 Protein ID WP_002887278.1
Coordinates 4756525..4756791 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLQ62_RS23005 (4754812) 4754812..4755933 + 1122 WP_012737335.1 cupin domain-containing protein -
CLQ62_RS23010 (4755981) 4755981..4756514 - 534 WP_014908042.1 type II toxin-antitoxin system toxin KacT Toxin
CLQ62_RS23015 (4756525) 4756525..4756791 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
CLQ62_RS23020 (4756894) 4756894..4758327 - 1434 WP_014908043.1 Cu(+)/Ag(+) sensor histidine kinase -
CLQ62_RS23025 (4758317) 4758317..4759000 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
CLQ62_RS23030 (4759172) 4759172..4760557 + 1386 WP_012737332.1 efflux transporter outer membrane subunit -
CLQ62_RS23035 (4760575) 4760575..4760919 + 345 WP_012737331.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19795.68 Da        Isoelectric Point: 5.2614

>T199660 WP_014908042.1 NZ_CP073285:c4756514-4755981 [Klebsiella pneumoniae subsp. ozaenae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T199660 NZ_CP097430:c4020972-4020685 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT199660 WP_002887278.1 NZ_CP073285:c4756791-4756525 [Klebsiella pneumoniae subsp. ozaenae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT199660 NZ_CP097430:c4020698-4020396 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332NV98

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References