Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4164711..4165330 Replicon chromosome
Accession NZ_CP073285
Organism Klebsiella pneumoniae subsp. ozaenae strain WCHKP030925

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CLQ62_RS20165 Protein ID WP_002892050.1
Coordinates 4165112..4165330 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag CLQ62_RS20160 Protein ID WP_002892066.1
Coordinates 4164711..4165085 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLQ62_RS20150 (4159863) 4159863..4161056 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CLQ62_RS20155 (4161079) 4161079..4164225 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
CLQ62_RS20160 (4164711) 4164711..4165085 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
CLQ62_RS20165 (4165112) 4165112..4165330 + 219 WP_002892050.1 HHA domain-containing protein Toxin
CLQ62_RS20170 (4165489) 4165489..4166055 + 567 WP_002892030.1 maltose O-acetyltransferase -
CLQ62_RS20175 (4166027) 4166027..4166167 - 141 WP_004147370.1 hypothetical protein -
CLQ62_RS20180 (4166188) 4166188..4166658 + 471 WP_002892026.1 YlaC family protein -
CLQ62_RS20185 (4166633) 4166633..4168084 - 1452 WP_014907971.1 PLP-dependent aminotransferase family protein -
CLQ62_RS20190 (4168185) 4168185..4168883 + 699 WP_002892021.1 GNAT family protein -
CLQ62_RS20195 (4168880) 4168880..4169020 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
CLQ62_RS20200 (4169020) 4169020..4169283 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T199659 WP_002892050.1 NZ_CP073285:4165112-4165330 [Klebsiella pneumoniae subsp. ozaenae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T199659 NZ_CP097430:3708007-3708285 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT199659 WP_002892066.1 NZ_CP073285:4164711-4165085 [Klebsiella pneumoniae subsp. ozaenae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT199659 NZ_CP097430:3707744-3708004 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References