Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 7266..7867 Replicon plasmid p5
Accession NZ_CP073271
Organism Lactiplantibacillus plantarum strain MNCW_1

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag KCZ48_RS15645 Protein ID WP_001748110.1
Coordinates 7523..7867 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A806J926
Locus tag KCZ48_RS15640 Protein ID WP_001748109.1
Coordinates 7266..7529 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KCZ48_RS15620 (KCZ48_15570) 2432..2632 + 201 WP_003643340.1 cold-shock protein -
KCZ48_RS15625 (KCZ48_15575) 2719..2961 + 243 WP_003646142.1 hypothetical protein -
KCZ48_RS15630 (KCZ48_15580) 3536..4060 + 525 WP_003646143.1 hypothetical protein -
KCZ48_RS15635 (KCZ48_15585) 6593..7180 - 588 WP_248847095.1 site-specific integrase -
KCZ48_RS15640 (KCZ48_15590) 7266..7529 + 264 WP_001748109.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
KCZ48_RS15645 (KCZ48_15595) 7523..7867 + 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KCZ48_RS15650 (KCZ48_15600) 8101..8888 + 788 WP_248847094.1 IS5 family transposase -
KCZ48_RS15655 (KCZ48_15605) 8978..9832 - 855 WP_021816930.1 transporter substrate-binding domain-containing protein -
KCZ48_RS15660 (KCZ48_15610) 9853..10593 - 741 WP_015639667.1 amino acid ABC transporter ATP-binding protein -
KCZ48_RS15665 (KCZ48_15615) 10586..11275 - 690 WP_003645002.1 amino acid ABC transporter permease -
KCZ48_RS15670 (KCZ48_15620) 11256..11918 - 663 WP_003645001.1 amino acid ABC transporter permease -
KCZ48_RS15675 (KCZ48_15625) 12317..12663 + 347 Protein_14 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..13244 13244


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T199639 WP_001748110.1 NZ_CP073271:7523-7867 [Lactiplantibacillus plantarum]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T199639 NZ_CP097426:c4243670-4243497 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.47 Da        Isoelectric Point: 5.7117

>AT199639 WP_001748109.1 NZ_CP073271:7266-7529 [Lactiplantibacillus plantarum]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT199639 NZ_CP097426:c4243994-4243698 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A806J926

References