Detailed information of TA system TA199638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1434747..1435663
• Replicon: chromosome
• Accession: NZ_CP073265
• Organism: Bacillus atrophaeus strain CNY01

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A7U5BWH7
• Locus tag: KCX77_RS07410
• Protein ID: WP_003327423.1
• Coordinates: 1434917..1435663 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: A0A837Y0B4
• Locus tag: KCX77_RS07405
• Protein ID: WP_010788357.1
• Coordinates: 1434747..1434917 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCX77_RS07370 (KCX77_07370)	1431502..1431831	+	330	WP_035175088.1	XkdW family protein	-
KCX77_RS07375 (KCX77_07375)	1431828..1431992	+	165	WP_061571316.1	XkdX family protein	-
KCX77_RS07380 (KCX77_07380)	1432069..1432917	+	849	WP_212064487.1	phage portal protein	-
KCX77_RS07385 (KCX77_07385)	1432991..1433269	+	279	WP_003327427.1	hemolysin XhlA family protein	-
KCX77_RS07390 (KCX77_07390)	1433282..1433545	+	264	WP_003327426.1	phage holin	-
KCX77_RS07395 (KCX77_07395)	1433558..1434451	+	894	WP_003327425.1	N-acetylmuramoyl-L-alanine amidase	-
KCX77_RS07400 (KCX77_07400)	1434527..1434649	-	123	WP_010788356.1	hypothetical protein	-
KCX77_RS07405 (KCX77_07405)	1434747..1434917	-	171	WP_010788357.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
KCX77_RS07410 (KCX77_07410)	1434917..1435663	-	747	WP_003327423.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
KCX77_RS07415 (KCX77_07415)	1435772..1436770	-	999	WP_003327422.1	inorganic phosphate transporter	-
KCX77_RS07420 (KCX77_07420)	1436783..1437400	-	618	WP_003327421.1	DUF47 domain-containing protein	-
KCX77_RS07425 (KCX77_07425)	1437689..1439005	-	1317	WP_003327418.1	amino acid permease	-
KCX77_RS07430 (KCX77_07430)	1439425..1440375	+	951	WP_212064488.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29110.66 Da
• Isoelectric Point: 4.5650

>T199638 WP_003327423.1 NZ_CP073265:c1435663-1434917 [Bacillus atrophaeus]
MLLFFQIMVWCIMAALGLYVYAVWRFEAKVKEKMFAIRKTWYLIFVIGAMVYWTYDPSSLFREWTNYLIVAVSFALIDAF
IFLSAYVKKLAGNELETDTREILEENNEMLHVYLDKLKTYQYLLKNEPIHVYYGSIEAYAEGIDKLLKTYADKMNLTASL
CEYSTQLEKDRLTEHMDDAFEVQSRLDRKDVYYDQHGKLALIPFTVQTRSYVIKLASDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGDG

Nucleotide

• Length: 747 bp

>T199638 NZ_CP097426:c4013276-4012989 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6605.59 Da
• Isoelectric Point: 11.0258

>AT199638 WP_010788357.1 NZ_CP073265:c1434917-1434747 [Bacillus atrophaeus]
MERAFQNDHSPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT199638 NZ_CP097426:c4013002-4012700 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A7U5BWH7

Antitoxin

• AlphaFold DB: A0A837Y0B4