Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 531675..532311 Replicon chromosome
Accession NZ_CP073265
Organism Bacillus atrophaeus strain CNY01

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag KCX77_RS02615 Protein ID WP_003156187.1
Coordinates 531961..532311 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A837XXL9
Locus tag KCX77_RS02610 Protein ID WP_003328141.1
Coordinates 531675..531956 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KCX77_RS02590 (KCX77_02590) 527972..528571 - 600 WP_010789631.1 rhomboid family intramembrane serine protease -
KCX77_RS02595 (KCX77_02595) 528670..529035 + 366 WP_061669621.1 holo-ACP synthase -
KCX77_RS02600 (KCX77_02600) 529203..530219 + 1017 WP_212064195.1 outer membrane lipoprotein carrier protein LolA -
KCX77_RS02605 (KCX77_02605) 530387..531556 + 1170 WP_063638785.1 alanine racemase -
KCX77_RS02610 (KCX77_02610) 531675..531956 + 282 WP_003328141.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
KCX77_RS02615 (KCX77_02615) 531961..532311 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KCX77_RS02620 (KCX77_02620) 532427..533245 + 819 WP_061572303.1 RsbT co-antagonist protein RsbRA -
KCX77_RS02625 (KCX77_02625) 533250..533615 + 366 WP_010789626.1 RsbT antagonist protein RsbS -
KCX77_RS02630 (KCX77_02630) 533618..534019 + 402 WP_003328144.1 serine/threonine-protein kinase RsbT -
KCX77_RS02635 (KCX77_02635) 534031..535038 + 1008 WP_063638786.1 PP2C family protein-serine/threonine phosphatase -
KCX77_RS02640 (KCX77_02640) 535098..535427 + 330 WP_003328146.1 anti-sigma factor antagonist RsbV -
KCX77_RS02645 (KCX77_02645) 535424..535906 + 483 WP_003328147.1 anti-sigma B factor RsbW -
KCX77_RS02650 (KCX77_02650) 535872..536660 + 789 WP_106047471.1 RNA polymerase sigma factor SigB -
KCX77_RS02655 (KCX77_02655) 536660..537259 + 600 WP_003328150.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T199637 WP_003156187.1 NZ_CP073265:531961-532311 [Bacillus atrophaeus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T199637 NZ_CP097426:3700310-3700588 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10451.83 Da        Isoelectric Point: 5.1903

>AT199637 WP_003328141.1 NZ_CP073265:531675-531956 [Bacillus atrophaeus]
MSESSATTEMMISLPEALVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT199637 NZ_CP097426:3700047-3700307 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB A0A837XXL9

References