Detailed information of TA system TA199635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 17961..18435
• Replicon: plasmid unnamed2
• Accession: NZ_CP073264
• Organism: Duffyella gerundensis strain AR

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: J9874_RS20030
• Protein ID: WP_225702154.1
• Coordinates: 18169..18435 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: J9874_RS20025
• Protein ID: WP_225702143.1
• Coordinates: 17961..18185 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J9874_RS20000 (J9874_03997)	13292..14050	-	759	WP_225702150.1	ABC transporter ATP-binding protein	-
J9874_RS20005 (J9874_03998)	14047..15084	-	1038	WP_225702151.1	iron ABC transporter permease	-
J9874_RS20010 (J9874_03999)	15084..16085	-	1002	WP_225702170.1	ABC transporter substrate-binding protein	-
J9874_RS20015 (J9874_04000)	16846..17205	+	360	WP_225702152.1	hypothetical protein	-
J9874_RS20020 (J9874_04001)	17255..17581	+	327	WP_225702153.1	NIPSNAP family protein	-
J9874_RS20025 (J9874_04002)	17961..18185	+	225	WP_225702143.1	DUF6290 family protein	Antitoxin
J9874_RS20030 (J9874_04003)	18169..18435	+	267	WP_225702154.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J9874_RS20035 (J9874_04004)	18551..18862	-	312	WP_225702155.1	hypothetical protein	-
J9874_RS20040 (J9874_04005)	19198..19866	+	669	WP_225702156.1	recombinase family protein	-
J9874_RS20045	19844..20257	+	414	WP_187493924.1	hypothetical protein	-
J9874_RS20050 (J9874_04007)	20285..21793	-	1509	WP_225702157.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
J9874_RS20055 (J9874_04008)	21793..22158	-	366	WP_187493926.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..107871	107871

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10240.80 Da
• Isoelectric Point: 8.9201

>T199635 WP_225702154.1 NZ_CP073264:18169-18435 [Duffyella gerundensis]
MVWTISYSERALKSLRKIDKQNARRIVDFMDLRVAVAADPRQSGKPLKGELGEFWRYRVGDYRILCEIGDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T199635 NZ_CP097426:3455562-3455780 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8379.32 Da
• Isoelectric Point: 4.6533

>AT199635 WP_225702143.1 NZ_CP073264:17961-18185 [Duffyella gerundensis]
MLAIRLSDEIEARLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEPVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT199635 NZ_CP097426:3455162-3455536 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG