Detailed information of TA system TA199632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2633017..2633642
• Replicon: chromosome
• Accession: NZ_CP073262
• Organism: Duffyella gerundensis strain AR

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A0U5L254
• Locus tag: J9874_RS12100
• Protein ID: WP_067428457.1
• Coordinates: 2633424..2633642 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: J9874_RS12095
• Protein ID: WP_067428460.1
• Coordinates: 2633017..2633391 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J9874_RS12065 (J9874_02414)	2629278..2629535	+	258	WP_067428465.1	type B 50S ribosomal protein L31	-
J9874_RS12070 (J9874_02415)	2629545..2629685	+	141	WP_071852488.1	type B 50S ribosomal protein L36	-
J9874_RS12075 (J9874_02416)	2629760..2630638	-	879	WP_067428464.1	metal ABC transporter substrate-binding protein	-
J9874_RS12080 (J9874_02417)	2630655..2631494	-	840	WP_067435179.1	metal ABC transporter permease	-
J9874_RS12085 (J9874_02418)	2631491..2632168	-	678	WP_225700929.1	ABC transporter ATP-binding protein	-
J9874_RS12090 (J9874_02419)	2632515..2632868	+	354	WP_067428462.1	hypothetical protein	-
J9874_RS12095 (J9874_02420)	2633017..2633391	+	375	WP_067428460.1	Hha toxicity modulator TomB	Antitoxin
J9874_RS12100 (J9874_02421)	2633424..2633642	+	219	WP_067428457.1	HHA domain-containing protein	Toxin
J9874_RS12110 (J9874_02422)	2634009..2634323	+	315	WP_225700930.1	MGMT family protein	-
J9874_RS12115 (J9874_02423)	2634350..2634907	-	558	WP_067435173.1	YbaY family lipoprotein	-
J9874_RS12120 (J9874_02424)	2635098..2635961	+	864	WP_067435170.1	acyl-CoA thioesterase II	-
J9874_RS12125 (J9874_02425)	2636023..2637312	-	1290	WP_067435167.1	ammonium transporter AmtB	-
J9874_RS12130 (J9874_02426)	2637342..2637680	-	339	WP_067428451.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8640.06 Da
• Isoelectric Point: 9.4825

>T199632 WP_067428457.1 NZ_CP073262:2633424-2633642 [Duffyella gerundensis]
MSDKTLTKTDYLMRLRRCRSIDTLERVIEKNKYELPDNELAIFYSAADHRLAELTMNKLYDKVPVSVWKFVR

Nucleotide

• Length: 219 bp

>T199632 NZ_CP097426:c2392297-2392121 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14474.08 Da
• Isoelectric Point: 4.5014

>AT199632 WP_067428460.1 NZ_CP073262:2633017-2633391 [Duffyella gerundensis]
MDEYSPKRHDMAQLKYLCESLHDDSMATLSDSHHGWVNDPTSASNLQLNDLIEHIASFTMNYKIKHVEDEELITQIDEYL
DDTFMLFSNYGVSAQDLQRWQRSAKRLFNIFAEECALLQPSHSF

Nucleotide

• Length: 375 bp

>AT199632 NZ_CP097426:c2392075-2391659 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0U5L254