Detailed information of TA system TA199629

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2487132..2488153
• Replicon: chromosome
• Accession: NZ_CP073248
• Organism: Proteus mirabilis strain N292

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: A0A8E4FRK4
• Locus tag: KCV17_RS11520
• Protein ID: WP_017827532.1
• Coordinates: 2487132..2487701 (-)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: KCV17_RS11525
• Protein ID: WP_004245528.1
• Coordinates: 2487701..2488153 (-)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCV17_RS11515	2484662..2487016	-	2355	WP_109828966.1	DNA polymerase II	-
KCV17_RS11520	2487132..2487701	-	570	WP_017827532.1	PIN domain-containing protein	Toxin
KCV17_RS11525	2487701..2488153	-	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
KCV17_RS11530	2488281..2488982	-	702	WP_004245527.1	thiamine ABC transporter ATP-binding protein ThiQ	-
KCV17_RS11535	2488975..2490555	-	1581	WP_104731821.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
KCV17_RS11540	2490555..2491520	-	966	WP_049195511.1	thiamine ABC transporter substrate binding subunit	-
KCV17_RS11545	2491837..2492367	+	531	WP_004245524.1	RNA pyrophosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21601.08 Da
• Isoelectric Point: 7.0180

>T199629 WP_017827532.1 NZ_CP073248:c2487701-2487132 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKGQAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKDPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T199629 NZ_CP097426:1972716-1972819 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT199629 WP_004245528.1 NZ_CP073248:c2488153-2487701 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT199629 NZ_CP097426:c1972857-1972586 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: B4F2H3