Detailed information of TA system TA199628

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-MqsA
• Location: 2047206..2047905
• Replicon: chromosome
• Accession: NZ_CP073248
• Organism: Proteus mirabilis strain N292

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B4EZ96
• Locus tag: KCV17_RS09610
• Protein ID: WP_004249093.1
• Coordinates: 2047519..2047905 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B4EZ97
• Locus tag: KCV17_RS09605
• Protein ID: WP_004246828.1
• Coordinates: 2047206..2047526 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCV17_RS09575	2042334..2042609	-	276	WP_004246820.1	DNA-directed RNA polymerase subunit omega	-
KCV17_RS09580	2042664..2043287	-	624	WP_004246821.1	guanylate kinase	-
KCV17_RS09585	2043589..2044206	-	618	WP_004246822.1	trimeric intracellular cation channel family protein	-
KCV17_RS09590	2044343..2045629	+	1287	WP_036919265.1	DUF3748 domain-containing protein	-
KCV17_RS09595	2045846..2046730	+	885	WP_012368526.1	endonuclease/exonuclease/phosphatase family protein	-
KCV17_RS09600	2046723..2047157	+	435	WP_004246827.1	hypothetical protein	-
KCV17_RS09605	2047206..2047526	-	321	WP_004246828.1	helix-turn-helix domain-containing protein	Antitoxin
KCV17_RS09610	2047519..2047905	-	387	WP_004249093.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KCV17_RS09615	2048091..2050163	-	2073	WP_036919263.1	glycine--tRNA ligase subunit beta	-
KCV17_RS09620	2050173..2051078	-	906	WP_004246832.1	glycine--tRNA ligase subunit alpha	-
KCV17_RS09625	2051329..2051910	+	582	WP_012368524.1	DNA-3-methyladenine glycosylase I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-125)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-95)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14294.64 Da
• Isoelectric Point: 10.0642

>T199628 WP_004249093.1 NZ_CP073248:c2047905-2047519 [Proteus mirabilis]
MVIYKTKMFKNSFKKITLTNAELIAIATEVLAGQFEADLGGGVIKKRACIQGKGKSSGIRTIIFYKQGNNLFFADGWKKS
SLSSKKTKEITDDELESYKDLAKDLFNANQNKIDKMIALGLLTEVKYD

Nucleotide

• Length: 387 bp

>T199628 NZ_CP097426:c1768341-1767967 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGTATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12126.21 Da
• Isoelectric Point: 10.4411

>AT199628 WP_004246828.1 NZ_CP073248:c2047526-2047206 [Proteus mirabilis]
MTDKMKEMLKFAQEMEDAGVTDGSTTKRIAARIKAKELQAKYKHPTPMTGEDIKRIREQNNISQSSLALILNMTKESVSK
WERNEIKPNGAALRLLHLIDKKILFI

Nucleotide

• Length: 321 bp

>AT199628 NZ_CP097426:c1768799-1768431 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGTGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: B4EZ96

Antitoxin

• AlphaFold DB: B4EZ97