Detailed information of TA system TA199627

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1745683..1747254
• Replicon: chromosome
• Accession: NZ_CP073248
• Organism: Proteus mirabilis strain N292

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B4F0T8
• Locus tag: KCV17_RS08280
• Protein ID: WP_012368637.1
• Coordinates: 1745941..1747254 (+)
• Length: 438 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: B4F0T9
• Locus tag: KCV17_RS08275
• Protein ID: WP_004249771.1
• Coordinates: 1745683..1745940 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCV17_RS08250	1741645..1741788	+	144	WP_004246509.1	50S ribosomal protein L34	-
KCV17_RS08255	1741806..1742165	+	360	WP_004246510.1	ribonuclease P protein component	-
KCV17_RS08260	1742129..1742389	+	261	WP_012368639.1	membrane protein insertion efficiency factor YidD	-
KCV17_RS08265	1742392..1744032	+	1641	WP_004246512.1	membrane protein insertase YidC	-
KCV17_RS08270	1744170..1745534	+	1365	WP_004246513.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-
KCV17_RS08275	1745683..1745940	+	258	WP_004249771.1	helix-turn-helix domain-containing protein	Antitoxin
KCV17_RS08280	1745941..1747254	+	1314	WP_012368637.1	type II toxin-antitoxin system HipA family toxin	Toxin
KCV17_RS08285	1747656..1748120	+	465	WP_004246516.1	DUF1097 domain-containing protein	-
KCV17_RS08290	1748305..1749234	+	930	WP_060554980.1	metallophosphoesterase	-
KCV17_RS08295	1749371..1751227	+	1857	WP_232091310.1	insecticidal delta-endotoxin Cry8Ea1 family protein	-
KCV17_RS08300	1751281..1752084	-	804	WP_060554978.1	energy transducer TonB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 438 a.a.
• Molecular weight: 49670.78 Da
• Isoelectric Point: 6.5023

>T199627 WP_012368637.1 NZ_CP073248:1745941-1747254 [Proteus mirabilis]
MASLDVYMNEYYVGIFTKKSSGAHSFQYTEDWVNQPGSRPISLSMPLQLQEYQGDCVYNFFDNLLPDNTEIRNRIVSRYN
ANSNQPFDLLAAIGADTVGALRLLPSGSTPADIKKIEYQKLNDIELEKILLGYKSNTPLGMLKEYTDFRISIAGAQEKTA
LLLKDNHWCVPHNNTPTTHIIKLPIGVIESHSYTMDLSNSVENEYLCTLIAKEFDLPVPHCSIIKTKNVKALAVERFDRK
YSSDKSWIIRLPQEDFCQILNIPSALKYENEGGPGIIDIMKYLLGSSNADQDRFHFMKAQVLFWLLAATDGHAKNFSVFI
NQEGRFHLTPLYDIMSIYPNFGGRGLNPRDAKLAMGLKASRGKKYAIEQIFPRHFYQTAEAVGFDPSLMSDILRYFYQEM
DDVIQRVKQQLPEDFPSEISNTILDGMKARAVRLANI

Nucleotide

• Length: 1314 bp

>T199627 NZ_CP097426:1750783-1751037 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9878.43 Da
• Isoelectric Point: 10.0139

>AT199627 WP_004249771.1 NZ_CP073248:1745683-1745940 [Proteus mirabilis]
MIIINAYQLSVHLKDVRLTKKLSQSKVAQKVGIRQDTVSNFELNPNSTKLETFFKLLSALNLEMEIHPKDAKNNLAHKSE
WKEEW

Nucleotide

• Length: 258 bp

>AT199627 NZ_CP097426:1750535-1750786 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: B4F0T8

Antitoxin

• AlphaFold DB: B4F0T9