Detailed information of TA system TA199625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 20003..20802
• Replicon: chromosome
• Accession: NZ_CP073248
• Organism: Proteus mirabilis strain N292

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A8E3RD10
• Locus tag: KCV17_RS00085
• Protein ID: WP_004247966.1
• Coordinates: 20003..20527 (-)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: B4EVS2
• Locus tag: KCV17_RS00090
• Protein ID: WP_004247964.1
• Coordinates: 20524..20802 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCV17_RS00070	16328..17269	-	942	WP_036918236.1	glyoxylate/hydroxypyruvate reductase GhrA	-
KCV17_RS00080	18222..19922	+	1701	WP_004247967.1	C4-dicarboxylic acid transporter DauA	-
KCV17_RS00085	20003..20527	-	525	WP_004247966.1	hypothetical protein	Toxin
KCV17_RS00090	20524..20802	-	279	WP_004247964.1	DUF1778 domain-containing protein	Antitoxin
KCV17_RS00095	20880..21809	-	930	WP_017628160.1	TIGR01212 family radical SAM protein	-
KCV17_RS00100	21817..22671	-	855	WP_004242689.1	3-deoxy-8-phosphooctulonate synthase	-
KCV17_RS00105	22729..23544	-	816	WP_004242688.1	invasion regulator SirB1	-
KCV17_RS00110	23522..24370	-	849	WP_004247962.1	peptide chain release factor N(5)-glutamine methyltransferase	-
KCV17_RS00115	24370..25452	-	1083	WP_004242680.1	peptide chain release factor 1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (5-87)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19192.12 Da
• Isoelectric Point: 9.3561

>T199625 WP_004247966.1 NZ_CP073248:c20527-20003 [Proteus mirabilis]
MTIPNWHEEAISKKHNRNGFNCGDAVLNQFLYRHARQNHENGSAKTYLAINNSNNIILGYYSLCPASIEFERTPELIKHG
LARHDIPVFKLARLATDLSVQGKGLGGQLLLAAGRRCLSVASELGGVALLIDAKNERVANWYISYGAIPLLDTPLSLLIS
FKTIYTALSMANKI

Nucleotide

• Length: 525 bp

>T199625 NZ_CP097426:957932-958267 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTCG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10252.82 Da
• Isoelectric Point: 5.1369

>AT199625 WP_004247964.1 NZ_CP073248:c20802-20524 [Proteus mirabilis]
MAQALVENSERVSIRITTEDKSLLVRAASLENTNLTEFVLRNIVPVARQVIENNERLKLSEKDTLRVMELLDNPPKPNAK
LLAAAFALPDNA

Nucleotide

• Length: 279 bp

>AT199625 NZ_CP097426:957684-957932 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGACTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: B4EVS2