Detailed information of TA system TA199624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3250639..3251288
• Replicon: chromosome
• Accession: NZ_CP073247
• Organism: Proteus mirabilis strain S62-3-2-2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B4EZB9
• Locus tag: KCV16_RS14955
• Protein ID: WP_012368534.1
• Coordinates: 3250869..3251288 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: B4EZC0
• Locus tag: KCV16_RS14950
• Protein ID: WP_012368535.1
• Coordinates: 3250639..3250872 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCV16_RS14925	3246350..3246829	-	480	WP_060555753.1	Hcp family type VI secretion system effector	-
KCV16_RS14930	3247279..3247566	-	288	WP_104731871.1	transposase	-
KCV16_RS14935	3247614..3247835	+	222	WP_060555751.1	hypothetical protein	-
KCV16_RS14940	3248052..3249260	-	1209	WP_060555750.1	DUF262 domain-containing protein	-
KCV16_RS14945	3250220..3250372	-	153	WP_172764486.1	hypothetical protein	-
KCV16_RS14950	3250639..3250872	+	234	WP_012368535.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KCV16_RS14955	3250869..3251288	+	420	WP_012368534.1	type II toxin-antitoxin system VapC family toxin	Toxin
KCV16_RS14960	3251513..3251836	+	324	WP_104731870.1	hypothetical protein	-
KCV16_RS14965	3251814..3252230	+	417	WP_046334846.1	hypothetical protein	-
KCV16_RS14970	3252882..3253499	+	618	WP_004246809.1	glucose-1-phosphatase	-
KCV16_RS14975	3253580..3254017	+	438	WP_004246810.1	D-aminoacyl-tRNA deacylase	-
KCV16_RS14980	3254041..3254955	+	915	WP_004249085.1	fatty acid biosynthesis protein FabY	-
KCV16_RS14985	3255131..3256009	+	879	WP_017628653.1	23S rRNA pseudouridine(2604) synthase RluF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (9-56)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15371.88 Da
• Isoelectric Point: 7.7288

>T199624 WP_012368534.1 NZ_CP073247:3250869-3251288 [Proteus mirabilis]
VKKVYMLDTNICSFIMREQPISLLEKLQKCVMNHDTIVISAITYSEMRFGAIGKKASPKHNRLVDAFCERVDAILAWDKA
AVDATTVIKKCLSDVGLPIGNNDSAIAGHAVAVNAILVTNNTREFSRVEGLKIEDWTHV

Nucleotide

• Length: 420 bp

>T199624 NZ_CP097426:820476-820883 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8890.06 Da
• Isoelectric Point: 4.4870

>AT199624 WP_012368535.1 NZ_CP073247:3250639-3250872 [Proteus mirabilis]
MRTRTVAVFKNGSNRAIRLPKDFDFDGVNELEISRDGDCIILRPVKPNWISLQNEEKADSDFLLERDDVIEEGRVIL

Nucleotide

• Length: 234 bp

>AT199624 NZ_CP097426:820229-820495 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: B4EZB9

Antitoxin

• AlphaFold DB: B4EZC0