Detailed information of TA system TA199621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3665050..3665699
• Replicon: chromosome
• Accession: NZ_CP073246
• Organism: Proteus mirabilis strain N639-2X

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B4EZB9
• Locus tag: KCV15_RS16765
• Protein ID: WP_012368534.1
• Coordinates: 3665280..3665699 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: B4EZC0
• Locus tag: KCV15_RS16760
• Protein ID: WP_012368535.1
• Coordinates: 3665050..3665283 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCV15_RS16750	3660696..3662105	-	1410	WP_004246801.1	glutamate--ammonia ligase	-
KCV15_RS16755	3662465..3664300	+	1836	WP_004246802.1	ribosome-dependent GTPase TypA	-
KCV15_RS16760	3665050..3665283	+	234	WP_012368535.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KCV15_RS16765	3665280..3665699	+	420	WP_012368534.1	type II toxin-antitoxin system VapC family toxin	Toxin
KCV15_RS16770	3665969..3666247	+	279	WP_230629709.1	hypothetical protein	-
KCV15_RS16775	3666225..3666641	+	417	WP_046334846.1	hypothetical protein	-
KCV15_RS16780	3667294..3667911	+	618	WP_020946398.1	glucose-1-phosphatase	-
KCV15_RS16785	3667991..3668428	+	438	WP_004246810.1	D-aminoacyl-tRNA deacylase	-
KCV15_RS16790	3668452..3669366	+	915	WP_004249085.1	fatty acid biosynthesis protein FabY	-
KCV15_RS16795	3669542..3670420	+	879	WP_046334847.1	23S rRNA pseudouridine(2604) synthase RluF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (9-56)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15371.88 Da
• Isoelectric Point: 7.7288

>T199621 WP_012368534.1 NZ_CP073246:3665280-3665699 [Proteus mirabilis]
VKKVYMLDTNICSFIMREQPISLLEKLQKCVMNHDTIVISAITYSEMRFGAIGKKASPKHNRLVDAFCERVDAILAWDKA
AVDATTVIKKCLSDVGLPIGNNDSAIAGHAVAVNAILVTNNTREFSRVEGLKIEDWTHV

Nucleotide

• Length: 420 bp

>T199621 NZ_CP097426:c605390-604926 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8890.06 Da
• Isoelectric Point: 4.4870

>AT199621 WP_012368535.1 NZ_CP073246:3665050-3665283 [Proteus mirabilis]
MRTRTVAVFKNGSNRAIRLPKDFDFDGVNELEISRDGDCIILRPVKPNWISLQNEEKADSDFLLERDDVIEEGRVIL

Nucleotide

• Length: 234 bp

>AT199621 NZ_CP097426:c605725-605390 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: B4EZB9

Antitoxin

• AlphaFold DB: B4EZC0