Detailed information of TA system TA199619

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 314643..315664
• Replicon: chromosome
• Accession: NZ_CP073246
• Organism: Proteus mirabilis strain N639-2X

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: KCV15_RS01360
• Protein ID: WP_249121604.1
• Coordinates: 314643..315146 (-)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: KCV15_RS01365
• Protein ID: WP_004245528.1
• Coordinates: 315212..315664 (-)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCV15_RS01355	312173..314527	-	2355	WP_196579518.1	DNA polymerase II	-
KCV15_RS01360	314643..315146	-	504	WP_249121604.1	PIN domain-containing protein	Toxin
KCV15_RS01365	315212..315664	-	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
KCV15_RS01370	315792..316493	-	702	WP_004245527.1	thiamine ABC transporter ATP-binding protein ThiQ	-
KCV15_RS01375	316486..318066	-	1581	WP_046334723.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
KCV15_RS01380	318066..319031	-	966	WP_020946190.1	thiamine ABC transporter substrate binding subunit	-
KCV15_RS01385	319348..319878	+	531	WP_211849873.1	RNA pyrophosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-91)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 19083.09 Da
• Isoelectric Point: 7.3736

>T199619 WP_249121604.1 NZ_CP073246:c315146-314643 [Proteus mirabilis]
MYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEPLICGLKLPDPNDRHVLAAAIKG
QAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKNPPMTVETFLGCLQKQKLPQFISHL
RAFQILL

Nucleotide

• Length: 504 bp

>T199619 NZ_CP097425:13620-14036 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT199619 WP_004245528.1 NZ_CP073246:c315664-315212 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT199619 NZ_CP097425:13393-13623 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: B4F2H3