Detailed information of TA system TA199618

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2371151..2371796
• Replicon: chromosome
• Accession: NZ_CP073245
• Organism: Proteus mirabilis strain S74-3-2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: KCV14_RS10580
• Protein ID: WP_060557166.1
• Coordinates: 2371611..2371796 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: B4ET85
• Locus tag: KCV14_RS10575
• Protein ID: WP_004247034.1
• Coordinates: 2371151..2371564 (-)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCV14_RS10545	2366909..2367403	+	495	WP_060557147.1	anti-virulence regulator CigR family protein	-
KCV14_RS10550	2367692..2367850	-	159	WP_004247025.1	hypothetical protein	-
KCV14_RS10555	2368299..2368475	+	177	WP_004247793.1	hypothetical protein	-
KCV14_RS10560	2368652..2369398	-	747	WP_060557148.1	class I SAM-dependent methyltransferase	-
KCV14_RS10565	2370265..2370843	+	579	WP_060557149.1	alpha/beta hydrolase	-
KCV14_RS10570	2370944..2371108	+	165	WP_239685734.1	hypothetical protein	-
KCV14_RS10575	2371151..2371564	-	414	WP_004247034.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KCV14_RS10580	2371611..2371796	-	186	WP_060557166.1	type II toxin-antitoxin system HicA family toxin	Toxin
KCV14_RS10585	2372639..2374225	+	1587	WP_060557150.1	hypothetical protein	-
KCV14_RS10590	2374463..2375638	-	1176	WP_060557151.1	site-specific integrase	-
KCV14_RS10595	2375640..2375837	-	198	WP_232464495.1	hypothetical protein	-
KCV14_RS10600	2376267..2376437	-	171	WP_020945461.1	hypothetical protein	-
KCV14_RS10605	2376465..2376644	-	180	WP_004250516.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2368299..2424381	56082

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 7034.29 Da
• Isoelectric Point: 11.1504

>T199618 WP_060557166.1 NZ_CP073245:c2371796-2371611 [Proteus mirabilis]
MKSSELIKLLEKNGWVLDRIKGSHHQFTHPNFSFVVTVPHPRKDLKKGTLNQILKSAKLKN

Nucleotide

• Length: 186 bp

>T199618 NZ_CP097423:c5390718-5390434 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 15251.98 Da
• Isoelectric Point: 4.3830

>AT199618 WP_004247034.1 NZ_CP073245:c2371564-2371151 [Proteus mirabilis]
MLYPAFIEIDEDGSASGWFPDIDGCIFAGDSIEEAHADARSAIDAHFELLSEKGLDIPSPKSQQEHLINSSGDYNNGIWL
LVDVDMDKYDGRAERINITLPHRLLSRIDTIVKTNPEYGSRSGFIATATRKELQKNI

Nucleotide

• Length: 414 bp

>AT199618 NZ_CP097423:c5390950-5390708 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: B4ET85