Detailed information of TA system TA199617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 567256..567905
• Replicon: chromosome
• Accession: NZ_CP073245
• Organism: Proteus mirabilis strain S74-3-2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: KCV14_RS02455
• Protein ID: WP_060556774.1
• Coordinates: 567256..567675 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: B4EZC0
• Locus tag: KCV14_RS02460
• Protein ID: WP_012368535.1
• Coordinates: 567672..567905 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCV14_RS02425	562532..563410	-	879	WP_060556778.1	23S rRNA pseudouridine(2604) synthase RluF	-
KCV14_RS02430	563586..564500	-	915	WP_060556777.1	fatty acid biosynthesis protein FabY	-
KCV14_RS02435	564524..564961	-	438	WP_060556776.1	D-aminoacyl-tRNA deacylase	-
KCV14_RS02440	565042..565659	-	618	WP_060556775.1	glucose-1-phosphatase	-
KCV14_RS02445	566313..566729	-	417	WP_049196823.1	hypothetical protein	-
KCV14_RS02450	566707..567111	-	405	WP_239685727.1	hypothetical protein	-
KCV14_RS02455	567256..567675	-	420	WP_060556774.1	type II toxin-antitoxin system VapC family toxin	Toxin
KCV14_RS02460	567672..567905	-	234	WP_012368535.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KCV14_RS02465	568667..570502	-	1836	WP_004246802.1	ribosome-dependent GTPase TypA	-
KCV14_RS02470	570862..572271	+	1410	WP_004246801.1	glutamate--ammonia ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (9-56)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15383.94 Da
• Isoelectric Point: 7.7288

>T199617 WP_060556774.1 NZ_CP073245:c567675-567256 [Proteus mirabilis]
VKKVYMLDTNICSFIMREQPISLLEKLQKCVMNHDTIVISAITYSEMRFGAIGKKASPKHNRLVDAFCERVDAILAWDKA
AVDATTVIKKCLSDVGLPIGNNDSAIAGHAVAVNAILVTNNIREFSRVEGLKIEDWTHV

Nucleotide

• Length: 420 bp

>T199617 NZ_CP097423:c5288874-5288341 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8890.06 Da
• Isoelectric Point: 4.4870

>AT199617 WP_012368535.1 NZ_CP073245:c567905-567672 [Proteus mirabilis]
MRTRTVAVFKNGSNRAIRLPKDFDFDGVNELEISRDGDCIILRPVKPNWISLQNEEKADSDFLLERDDVIEEGRVIL

Nucleotide

• Length: 234 bp

>AT199617 NZ_CP097423:c5289151-5288885 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: B4EZC0