Detailed information of TA system TA199616

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 232144..233873
• Replicon: chromosome
• Accession: NZ_CP073245
• Organism: Proteus mirabilis strain S74-3-2

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KCV14_RS00995
• Protein ID: WP_060556490.1
• Coordinates: 232144..233514 (+)
• Length: 457 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KCV14_RS01000
• Protein ID: WP_060556489.1
• Coordinates: 233511..233873 (+)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCV14_RS00980	227805..228788	-	984	WP_060556493.1	ABC transporter permease	-
KCV14_RS00985	228773..230356	-	1584	WP_060556492.1	ABC transporter substrate-binding protein	-
KCV14_RS00990	230757..231749	-	993	WP_060556491.1	glycosyltransferase	-
KCV14_RS00995	232144..233514	+	1371	WP_060556490.1	HipA domain-containing protein	Toxin
KCV14_RS01000	233511..233873	+	363	WP_060556489.1	helix-turn-helix transcriptional regulator	Antitoxin
KCV14_RS01005	234013..234513	-	501	WP_004245684.1	hydrogenase maturation peptidase HycI	-
KCV14_RS01010	234510..234920	-	411	WP_004245685.1	formate hydrogenlyase maturation HycH family protein	-
KCV14_RS01015	234913..235695	-	783	WP_060556488.1	NADH-quinone oxidoreductase subunit B family protein	-
KCV14_RS01020	235692..236246	-	555	WP_004245688.1	hydrogenase 4 subunit H	-
KCV14_RS01025	236258..237991	-	1734	WP_060556487.1	hydrogenase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (174-415)

Antitoxin

• Cro/C1-type helix-turn-helix domain (46-101)

Sequences

Toxin

Protein

• Length: 457 a.a.
• Molecular weight: 52482.38 Da
• Isoelectric Point: 4.7776

>T199616 WP_060556490.1 NZ_CP073245:232144-233514 [Proteus mirabilis]
MEKITLQAFLNQEWRDIAIINFPNDGHLALENQYDITYPITELAYESDYSIDYLFRDDHHAVSLNHPVSLFFDDKGSPGW
FKFLDDIVPSGASRRYWVQYLDIAGLSIAQQNYQLLRNGTISPIGNLRVKESLPHLSTLNNDLFFTIDEVKNRSSDFLEY
AQRKGAAAGGATGAGGEAPKLLLRCSHDEKIWIDTYQNDISNHDRYYLVKFPRGRRTSVDCNILRTEYAFYHELTAMGFN
TIPIEGMRLEEGDNYPSLWLPRFDIQIENNQVIRLAMESVYSILNKASGTTLYHEAVIREIIEKINQSNMVTEFGFQFNI
TNFIIEWVRRDLLNIIFGNSDNHGRNTSFIRKDNAIYLAPIYDFAPMKADPEGIVRTTKWQSYEMGGEFDFIGIANSLAD
LIPQEQLLEALRETALQLLDLKERLKERGVPEQILTMPTFGFDFIPDKLARWGLLP

Nucleotide

• Length: 1371 bp

>T199616 NZ_CP097423:4674382-4674600 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13542.07 Da
• Isoelectric Point: 10.6882

>AT199616 WP_060556489.1 NZ_CP073245:233511-233873 [Proteus mirabilis]
MNNIDNKITKLPLRASNNRSLIATIDRKKTIEKIMLSLLLEEISQGKALKILRLNILGLKQDAYAKLVNISRKTLSDIEN
DKGNYSIDIINKAFKPFGLKIGLVPATSHMLLSILKNHYQ

Nucleotide

• Length: 363 bp

>AT199616 NZ_CP097423:4673981-4674355 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG