Detailed information of TA system TA199609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 3491415..3493051
• Replicon: chromosome
• Accession: NZ_CP073243
• Organism: Acinetobacter seifertii strain S63-3

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KCV10_RS16400
• Protein ID: WP_212511357.1
• Coordinates: 3491415..3492746 (+)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: N8S5T2
• Locus tag: KCV10_RS16405
• Protein ID: WP_004701524.1
• Coordinates: 3492743..3493051 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCV10_RS16380	3486415..3488001	+	1587	WP_212511353.1	GH3 auxin-responsive promoter family protein	-
KCV10_RS16385	3487998..3489326	+	1329	WP_212511354.1	TolC family protein	-
KCV10_RS16390	3489319..3490482	+	1164	WP_212511355.1	hypothetical protein	-
KCV10_RS16395	3490681..3491121	-	441	WP_212511356.1	HD domain-containing protein	-
KCV10_RS16400	3491415..3492746	+	1332	WP_212511357.1	HipA domain-containing protein	Toxin
KCV10_RS16405	3492743..3493051	+	309	WP_004701524.1	helix-turn-helix transcriptional regulator	Antitoxin
KCV10_RS16410	3493774..3494322	+	549	WP_212511358.1	TetR/AcrR family transcriptional regulator	-
KCV10_RS16415	3494534..3495442	+	909	WP_212511359.1	hypothetical protein	-
KCV10_RS16420	3495561..3496757	+	1197	WP_212511360.1	hypothetical protein	-
KCV10_RS16425	3496848..3497066	-	219	WP_212511361.1	hypothetical protein	-
KCV10_RS16430	3497244..3497762	+	519	WP_212511362.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (167-359)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-83)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 51036.92 Da
• Isoelectric Point: 5.0501

>T199609 WP_212511357.1 NZ_CP073243:3491415-3492746 [Acinetobacter seifertii]
MKKLTIQALLNKNWLDIAELKLLEPKLGSASASELEYELDYAIQYSDRRDEHACSLSLPVQILIKHESNTWFGFLDDIVP
SGAARRYWVNYLDLQRLTHAEQESILLEKGGMAPVGNLRIKEALPPLNPESTLHLRYFSKDDVAERNIDFLEYAQQMGAI
SGGATGAGGEAPKLLIRVSPEQQVWIDTYQEQFDQPDQHYLVKFPRNKRSEIDCDILRAEYYYYQILNELGFNTIETTQM
QLIEGTKYPSLWLPRFDTEWRDQSWHRHGLESVYSVLNKSSGSHLNHFEVIEELCRLLTSIDSDFDASQFVCEWLQRDLL
NIIFGNSDNHGRNTSFLKKSGKIYLSPIYDFAPMKADPEVVTRSTTWGSPYEEGGEYRWLQITQKLATWCDPAVSLATLK
TLANNLLGLRQRLVDKGVPKQIIEMPALSFDYIEAKLKRWELL

Nucleotide

• Length: 1332 bp

>T199609 NZ_CP097423:760675-761043 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11590.52 Da
• Isoelectric Point: 9.9701

>AT199609 WP_004701524.1 NZ_CP073243:3492743-3493051 [Acinetobacter seifertii]
MNIKNQTQDRQQVLIDLYKQYLLSEITLGQLLSYLRKNVLGLSQEQYATLVGISRRTLTDIEQDKGKLTQSVLDKVFKPL
GLKAGLVPTHEHIVSKIIKPSE

Nucleotide

• Length: 309 bp

>AT199609 NZ_CP097423:760457-760678 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: N8S5T2