Detailed information of TA system TA199605

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 23970..24774
• Replicon: plasmid pkle02
• Accession: NZ_CP073237
• Organism: Klebsiella pasteurii strain Sb-24

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: KCG39_RS27400
• Protein ID: WP_211811657.1
• Coordinates: 23970..24497 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: KCG39_RS27405
• Protein ID: WP_048264996.1
• Coordinates: 24505..24774 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCG39_RS27370 (KCG39_27370)	19077..19787	-	711	WP_211811655.1	hypothetical protein	-
KCG39_RS27375 (KCG39_27375)	19887..20361	-	475	Protein_30	transposase	-
KCG39_RS27380 (KCG39_27380)	20476..20823	-	348	WP_142884384.1	hypothetical protein	-
KCG39_RS27385 (KCG39_27385)	21046..21363	+	318	Protein_32	AraC family transcriptional regulator	-
KCG39_RS27395 (KCG39_27395)	23023..23886	-	864	WP_211811656.1	hypothetical protein	-
KCG39_RS27400 (KCG39_27400)	23970..24497	-	528	WP_211811657.1	GNAT family N-acetyltransferase	Toxin
KCG39_RS27405 (KCG39_27405)	24505..24774	-	270	WP_048264996.1	DUF1778 domain-containing protein	Antitoxin
KCG39_RS27860	25250..25339	+	90	Protein_37	transposase	-
KCG39_RS27410 (KCG39_27410)	25349..25777	-	429	Protein_38	transposase	-
KCG39_RS27415 (KCG39_27415)	26041..26523	+	483	Protein_39	integrase core domain-containing protein	-
KCG39_RS27420 (KCG39_27420)	26968..28078	+	1111	Protein_40	IS3 family transposase	-
KCG39_RS27425 (KCG39_27425)	28268..29284	-	1017	WP_211811658.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..80069	80069
	-	inside	IScluster/Tn	-	-	21432..27884	6452

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (54-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 20118.15 Da
• Isoelectric Point: 5.7389

>T199605 WP_211811657.1 NZ_CP073237:c24497-23970 [Klebsiella pasteurii]
VMQELVIEMINDEFDYDIGEFDCGEETLNIFLKDHLKRQHDGQILKGYVLVTKEQKPKLLGYYTLSGSCFERKMLPSKTQ
QRKIPYQNAPSITLGRLAIDKSIQGQGWGELLVTHAMNVVWDASKAVGIYGLFVEALNDKAKRFYTRLDFIQLVGENSNL
LFYPTKSIEKIFTEE

Nucleotide

• Length: 528 bp

>T199605 NZ_CP097420:c57279-56797 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10148.65 Da
• Isoelectric Point: 10.1733

>AT199605 WP_048264996.1 NZ_CP073237:c24774-24505 [Klebsiella pasteurii]
MSALRKQRIDLRLTDDDKSLIEEAAAMSNQTITQFVVASASERAAEVIEQHRRMVLNEESWNLVMDAIANPAAPNERLKR
AAKRLQKMR

Nucleotide

• Length: 270 bp

>AT199605 NZ_CP097420:c57533-57267 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA