Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3028633..3029290 Replicon chromosome
Accession NZ_CP073236
Organism Klebsiella pasteurii strain Sb-24

Toxin (Protein)


Gene name cptA Uniprot ID H3N294
Locus tag KCG39_RS14160 Protein ID WP_004124948.1
Coordinates 3028633..3029043 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID H3N295
Locus tag KCG39_RS14165 Protein ID WP_004124953.1
Coordinates 3029024..3029290 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KCG39_RS14140 (KCG39_14140) 3024610..3026343 - 1734 WP_004124937.1 single-stranded-DNA-specific exonuclease RecJ -
KCG39_RS14145 (KCG39_14145) 3026349..3027062 - 714 WP_049115165.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KCG39_RS14150 (KCG39_14150) 3027085..3027981 - 897 WP_004124942.1 site-specific tyrosine recombinase XerD -
KCG39_RS14155 (KCG39_14155) 3028103..3028624 + 522 WP_004124946.1 flavodoxin FldB -
KCG39_RS14160 (KCG39_14160) 3028633..3029043 - 411 WP_004124948.1 protein YgfX Toxin
KCG39_RS14165 (KCG39_14165) 3029024..3029290 - 267 WP_004124953.1 FAD assembly factor SdhE Antitoxin
KCG39_RS14170 (KCG39_14170) 3029535..3030518 + 984 WP_004124956.1 tRNA-modifying protein YgfZ -
KCG39_RS14175 (KCG39_14175) 3030691..3033489 + 2799 WP_004124960.1 transcriptional regulator DagR -
KCG39_RS14180 (KCG39_14180) 3033610..3034035 + 426 WP_139529490.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16089.88 Da        Isoelectric Point: 10.9455

>T199600 WP_004124948.1 NZ_CP073236:c3029043-3028633 [Klebsiella pasteurii]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLVLPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWDIIGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDAGEWRDLRRLVLQKPTQE

Download         Length: 411 bp

>T199600 NZ_CP097419:2393718-2394002 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10411.88 Da        Isoelectric Point: 5.1296

>AT199600 WP_004124953.1 NZ_CP073236:c3029290-3029024 [Klebsiella pasteurii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDGDKALFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT199600 NZ_CP097419:2393486-2393728 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB H3N294

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3L9

References