Detailed information of TA system TA199591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 19982..20475
• Replicon: plasmid pGXG07-2
• Accession: NZ_CP073211
• Organism: Xanthomonas citri pv. mangiferaeindicae strain GXG07

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: XCM_RS22790
• Protein ID: WP_003491151.1
• Coordinates: 20149..20475 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: XCM_RS22785
• Protein ID: WP_017157755.1
• Coordinates: 19982..20152 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XCM_RS22760 (XCM_22570)	15680..16702	+	1023	WP_082340387.1	P-type DNA transfer ATPase VirB11	-
XCM_RS22765 (XCM_22575)	16713..17591	+	879	WP_033483303.1	lytic transglycosylase domain-containing protein	-
XCM_RS22770 (XCM_22580)	17717..18136	+	420	WP_011052981.1	hypothetical protein	-
XCM_RS22775 (XCM_22585)	18213..18599	+	387	WP_003491149.1	hypothetical protein	-
XCM_RS22780 (XCM_22595)	18730..19817	+	1088	WP_227072357.1	IS3-like element ISXac2 family transposase	-
XCM_RS22785 (XCM_22600)	19982..20152	+	171	WP_017157755.1	antitoxin MazE family protein	Antitoxin
XCM_RS22790 (XCM_22605)	20149..20475	+	327	WP_003491151.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
XCM_RS22795 (XCM_22610)	20528..23521	-	2994	WP_227072358.1	MobF family relaxase	-
XCM_RS22800 (XCM_22615)	23535..25109	-	1575	WP_029221893.1	type IV secretion system DNA-binding domain-containing protein	-
XCM_RS22805 (XCM_22620)	25113..25286	-	174	Protein_30	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..30169	30169

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-105)

Antitoxin

• Antitoxin MazE-like (2-54)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11690.73 Da
• Isoelectric Point: 9.2550

>T199591 WP_003491151.1 NZ_CP073211:20149-20475 [Xanthomonas citri pv. mangiferaeindicae]
MMRGDFVTIAMQGDFGKPRPALVIQADQFDAHTTVTVLPVTSTLVAAPLLRITIHPSTDNGLQKPSQVMVDKAMTVKRDK
VGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T199591 NZ_CP097415:c4966477-4965560 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCAATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6518.34 Da
• Isoelectric Point: 4.1476

>AT199591 WP_017157755.1 NZ_CP073211:19982-20152 [Xanthomonas citri pv. mangiferaeindicae]
MAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMADTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 171 bp

>AT199591 NZ_CP097415:c4967241-4966483 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA