Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 15468..16009 Replicon plasmid pGXG07-1
Accession NZ_CP073210
Organism Xanthomonas citri pv. mangiferaeindicae strain GXG07

Toxin (Protein)


Gene name mazF Uniprot ID A0A220WPB1
Locus tag XCM_RS22315 Protein ID WP_005917856.1
Coordinates 15686..16009 (+) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A220WPJ3
Locus tag XCM_RS22310 Protein ID WP_005917854.1
Coordinates 15468..15689 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
XCM_RS22280 (XCM_22110) 10530..11552 + 1023 WP_005931776.1 P-type DNA transfer ATPase VirB11 -
XCM_RS22285 (XCM_22115) 11563..12450 + 888 WP_033483242.1 lytic transglycosylase domain-containing protein -
XCM_RS22290 12692..12988 + 297 WP_126925283.1 hypothetical protein -
XCM_RS22295 (XCM_22120) 13092..13451 + 360 WP_033483239.1 hypothetical protein -
XCM_RS22300 (XCM_22125) 13520..14542 - 1023 WP_033483240.1 ParA family protein -
XCM_RS22305 (XCM_22135) 14697..15269 - 573 WP_003490825.1 recombinase family protein -
XCM_RS22310 (XCM_22140) 15468..15689 + 222 WP_005917854.1 antitoxin MazE family protein Antitoxin
XCM_RS22315 (XCM_22145) 15686..16009 + 324 WP_005917856.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
XCM_RS22320 (XCM_22150) 16035..16322 + 288 WP_029220894.1 hypothetical protein -
XCM_RS22325 (XCM_22155) 16329..17516 + 1188 WP_003490828.1 zeta toxin family protein -
XCM_RS22330 (XCM_22160) 17702..17986 - 285 WP_003490829.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..79634 79634
- flank IS/Tn - - 14697..15269 572


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-103)

Antitoxin

Antitoxin MazE-like

(9-72)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11513.56 Da        Isoelectric Point: 6.2913

>T199590 WP_005917856.1 NZ_CP073210:15686-16009 [Xanthomonas citri pv. mangiferaeindicae]
MTQRGDLVTVALPGAYGKPRPALVIQSDLLAELESVLVCPITSDLRDAMFRVTVEPSLTNGLQKISQVMVDKTAPIPRAK
VSEPIGTLDAERMRAVERALMLVTGLA

Download         Length: 324 bp

>T199590 NZ_CP097415:c4805381-4805097 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8240.23 Da        Isoelectric Point: 8.1023

>AT199590 WP_005917854.1 NZ_CP073210:15468-15689 [Xanthomonas citri pv. mangiferaeindicae]
MPRTHNPTNKVTRHRDRLRAAGYRPVQFWVHDTRSPELAAMLSRQCRSLHGDPTETDAIGFGEAAAGLIEGWE

Download         Length: 222 bp

>AT199590 NZ_CP097415:c4805613-4805371 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGGTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A220WPB1

Antitoxin

Source ID Structure
AlphaFold DB A0A220WPJ3

References