Detailed information of TA system TA199581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/RHH(antitoxin)
• Location: 3786676..3787241
• Replicon: chromosome
• Accession: NZ_CP073110
• Organism: Agrobacterium rhizogenes strain A4

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: EML492_RS18755
• Protein ID: WP_198929354.1
• Coordinates: 3786996..3787241 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B9J6S6
• Locus tag: EML492_RS18750
• Protein ID: WP_007702948.1
• Coordinates: 3786676..3786960 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EML492_RS18735 (EML492_18735)	3782484..3784121	+	1638	WP_007702958.1	ABC transporter ATP-binding protein	-
EML492_RS18740 (EML492_18740)	3784133..3785092	+	960	WP_007702954.1	glyoxylate/hydroxypyruvate reductase A	-
EML492_RS18745 (EML492_18745)	3785284..3786588	+	1305	WP_034519865.1	nicotinate phosphoribosyltransferase	-
EML492_RS18750 (EML492_18750)	3786676..3786960	+	285	WP_007702948.1	hypothetical protein	Antitoxin
EML492_RS18755 (EML492_18755)	3786996..3787241	+	246	WP_198929354.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EML492_RS18760 (EML492_18760)	3787252..3788208	-	957	WP_034519868.1	hypothetical protein	-
EML492_RS18765 (EML492_18765)	3788387..3790561	+	2175	WP_034519870.1	mechanosensitive ion channel	-
EML492_RS18770 (EML492_18770)	3790646..3791716	-	1071	WP_034519875.1	LacI family DNA-binding transcriptional regulator	-
EML492_RS18775 (EML492_18775)	3791856..3792155	+	300	WP_034519877.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-78)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9244.71 Da
• Isoelectric Point: 10.0946

>T199581 WP_198929354.1 NZ_CP073110:3786996-3787241 [Agrobacterium rhizogenes]
MRQFLQPKNAASAKKASTKIIQTIQMLSNQPDMGRPVEDLHQGLRELIVKFGRDGYVVLYRYIGDEVLIAAIRHGREDGY
K

Nucleotide

• Length: 246 bp

>T199581 NZ_CP097415:323452-323820 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10784.99 Da
• Isoelectric Point: 4.4877

>AT199581 WP_007702948.1 NZ_CP073110:3786676-3786960 [Agrobacterium rhizogenes]
MSSALKASVKIDPEVNERLEKLAASRHRQPDLLLKEAVEQYVDREEKRDSLLRDGRLSLDEYEATGLHVTGDEVIAWLET
WGDEDEKAPPECHR

Nucleotide

• Length: 285 bp

>AT199581 NZ_CP097415:323234-323455 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A5M8UMH1