Detailed information of TA system TA199578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 1065530..1065998
• Replicon: chromosome
• Accession: NZ_CP073110
• Organism: Agrobacterium rhizogenes strain A4

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: EML492_RS05345
• Protein ID: WP_234711648.1
• Coordinates: 1065765..1065998 (+)
• Length: 78 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A546ZEV1
• Locus tag: EML492_RS05340
• Protein ID: WP_034518680.1
• Coordinates: 1065530..1065775 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EML492_RS05320 (EML492_05320)	1060703..1061323	-	621	WP_165586802.1	glycerol-3-phosphate 1-O-acyltransferase PlsY	-
EML492_RS05325 (EML492_05325)	1061353..1062642	-	1290	WP_034518688.1	dihydroorotase	-
EML492_RS05330 (EML492_05330)	1062639..1063595	-	957	WP_034518686.1	aspartate carbamoyltransferase catalytic subunit	-
EML492_RS05335 (EML492_05335)	1063778..1065436	+	1659	WP_034518683.1	acyl-CoA dehydrogenase family protein	-
EML492_RS05340 (EML492_05340)	1065530..1065775	+	246	WP_034518680.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
EML492_RS05345 (EML492_05345)	1065765..1065998	+	234	WP_234711648.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EML492_RS05350 (EML492_05350)	1066129..1066746	+	618	WP_034518677.1	GNAT family N-acetyltransferase	-
EML492_RS05355 (EML492_05355)	1066850..1067509	+	660	WP_034518676.1	TetR/AcrR family transcriptional regulator	-
EML492_RS05360 (EML492_05360)	1067639..1067812	+	174	WP_165586803.1	hypothetical protein	-
EML492_RS05365 (EML492_05365)	1067809..1068141	+	333	WP_007692017.1	DUF6105 family protein	-
EML492_RS05370 (EML492_05370)	1068078..1068572	-	495	WP_007692016.1	Holliday junction resolvase RuvX	-
EML492_RS05375 (EML492_05375)	1068689..1069399	+	711	WP_034518669.1	metal-dependent hydrolase	-
EML492_RS05380 (EML492_05380)	1069467..1069754	+	288	WP_034518667.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-77)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 78 a.a.
• Molecular weight: 8783.88 Da
• Isoelectric Point: 6.4603

>T199578 WP_234711648.1 NZ_CP073110:1065765-1065998 [Agrobacterium rhizogenes]
MASKSGRYVLSPLAEADLEEIWRYTAENWSVKQAETYHAGILDAFEGLASGLKVGRYADIREGYFKYAISSHVIYYR

Nucleotide

• Length: 234 bp

>T199578 NZ_CP097411:c4968000-4967083 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCAATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8752.80 Da
• Isoelectric Point: 7.2019

>AT199578 WP_034518680.1 NZ_CP073110:1065530-1065775 [Agrobacterium rhizogenes]
MARNTSVSLGDHFNTFIDAQVQTGRYGSASDVVRAGLRLLEEHEAKVKALQGALIAGEESGKPTPFDNAAFPKRMRATYG
E

Nucleotide

• Length: 246 bp

>AT199578 NZ_CP097411:c4968764-4968006 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A546ZEV1