Detailed information of TA system TA199577

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 50784..51425
• Replicon: plasmid unnamed1
• Accession: NZ_CP073106
• Organism: Stutzerimonas stutzeri strain F2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: KCX70_RS23065
• Protein ID: WP_088192771.1
• Coordinates: 50784..50966 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KCX70_RS23070
• Protein ID: WP_088192770.1
• Coordinates: 51003..51425 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCX70_RS23035 (KCX70_23035)	46357..46713	+	357	WP_212620418.1	hypothetical protein	-
KCX70_RS23040 (KCX70_23040)	46728..47243	+	516	WP_212620419.1	hypothetical protein	-
KCX70_RS23045 (KCX70_23045)	47594..48079	+	486	WP_212620420.1	hypothetical protein	-
KCX70_RS23050 (KCX70_23050)	48096..48659	+	564	WP_249121755.1	hypothetical protein	-
KCX70_RS23055 (KCX70_23055)	48734..48931	+	198	WP_088192775.1	hypothetical protein	-
KCX70_RS23060 (KCX70_23060)	49551..50516	-	966	WP_212620421.1	DNA-binding protein	-
KCX70_RS23065 (KCX70_23065)	50784..50966	+	183	WP_088192771.1	type II toxin-antitoxin system HicA family toxin	Toxin
KCX70_RS23070 (KCX70_23070)	51003..51425	+	423	WP_088192770.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KCX70_RS23075 (KCX70_23075)	51437..51901	+	465	WP_212620422.1	hypothetical protein	-
KCX70_RS23080 (KCX70_23080)	52040..52738	-	699	WP_249121756.1	hypothetical protein	-
KCX70_RS23085 (KCX70_23085)	52704..53243	-	540	WP_088192768.1	hypothetical protein	-
KCX70_RS23090 (KCX70_23090)	53492..55705	+	2214	WP_212620423.1	hypothetical protein	-
KCX70_RS23095 (KCX70_23095)	55737..56264	+	528	WP_212620424.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..86675	86675

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6909.97 Da
• Isoelectric Point: 10.4140

>T199577 WP_088192771.1 NZ_CP073106:50784-50966 [Stutzerimonas stutzeri]
MKYSEFRRWLKAQGVSFEPAKGSHFKAYFGDRQTIVPDHGSKEIGEGLRKKILKDLGLKE

Nucleotide

• Length: 183 bp

>T199577 NZ_CP097411:c4806904-4806620 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15127.37 Da
• Isoelectric Point: 4.7323

>AT199577 WP_088192770.1 NZ_CP073106:51003-51425 [Stutzerimonas stutzeri]
MFDYPVIVHQEDGCVWISSDDVPELNSAGDNEAEALLDAVDGLESALSLYVDQRRAVPLPSPAKPGQPVVRLPALTAAKV
ALWNAMCAQGVGKAEMARRLGVNRPQVDRLVDLLHRSKIEQVEHALAILGQRLEISVIAA

Nucleotide

• Length: 423 bp

>AT199577 NZ_CP097411:c4807136-4806894 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGGTAAGGTGAGTCTGGATGACCTA
TGA