Detailed information of TA system TA199569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 4818290..4818898
• Replicon: chromosome
• Accession: NZ_CP073082
• Organism: Pseudomonas aeruginosa strain WTJH17

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: A0A1B1XUZ2
• Locus tag: KCG37_RS22310
• Protein ID: WP_003123043.1
• Coordinates: 4818290..4818637 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: KCG37_RS22315
• Protein ID: WP_003114155.1
• Coordinates: 4818647..4818898 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCG37_RS22280 (KCG37_22280)	4813710..4813922	+	213	WP_003098360.1	cysteine-rich CWC family protein	-
KCG37_RS22285 (KCG37_22285)	4813922..4814614	+	693	WP_003098362.1	16S rRNA pseudouridine(516) synthase	-
KCG37_RS22290 (KCG37_22290)	4814750..4815793	+	1044	WP_003098363.1	L,D-transpeptidase	-
KCG37_RS22295 (KCG37_22295)	4815873..4816610	+	738	WP_003085453.1	murein L,D-transpeptidase catalytic domain family protein	-
KCG37_RS22300 (KCG37_22300)	4817061..4817963	+	903	WP_003123042.1	(R)-3-hydroxydecanoyl-ACP:CoA transacylase	-
KCG37_RS22310 (KCG37_22310)	4818290..4818637	-	348	WP_003123043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KCG37_RS22315 (KCG37_22315)	4818647..4818898	-	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KCG37_RS22320 (KCG37_22320)	4819112..4820095	-	984	WP_003123044.1	tyrosine-type recombinase/integrase	-
KCG37_RS22325 (KCG37_22325)	4820095..4821387	-	1293	WP_003123045.1	hypothetical protein	-
KCG37_RS22330 (KCG37_22330)	4822037..4822426	-	390	WP_031687489.1	hypothetical protein	-
KCG37_RS22335 (KCG37_22335)	4822419..4822634	-	216	WP_003123046.1	hypothetical protein	-
KCG37_RS22340 (KCG37_22340)	4822657..4822872	-	216	WP_003123047.1	hypothetical protein	-
KCG37_RS32035	4822990..4823259	+	270	WP_078451377.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12990.74 Da
• Isoelectric Point: 4.4212

>T199569 WP_003123043.1 NZ_CP073082:c4818637-4818290 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAAFQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T199569 NZ_CP097411:349413-349760 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT199569 WP_003114155.1 NZ_CP073082:c4818898-4818647 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT199569 NZ_CP097411:349760-350059 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A1B1XUZ2

Antitoxin

• AlphaFold DB: A0A0B0C355