Detailed information of TA system TA199568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4500374..4501632
• Replicon: chromosome
• Accession: NZ_CP073082
• Organism: Pseudomonas aeruginosa strain WTJH17

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: KCG37_RS20720
• Protein ID: WP_003123189.1
• Coordinates: 4500374..4501129 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: KCG37_RS20725
• Protein ID: WP_003086329.1
• Coordinates: 4501264..4501632 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCG37_RS20710 (KCG37_20710)	4495547..4498090	+	2544	WP_003123188.1	acyl-homoserine-lactone acylase QuiP	-
KCG37_RS20715 (KCG37_20715)	4498245..4499606	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
KCG37_RS20720 (KCG37_20720)	4500374..4501129	-	756	WP_003123189.1	RES family NAD+ phosphorylase	Toxin
KCG37_RS20725 (KCG37_20725)	4501264..4501632	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
KCG37_RS20730 (KCG37_20730)	4501705..4502991	-	1287	WP_003123190.1	FAD-binding oxidoreductase	-
KCG37_RS20735 (KCG37_20735)	4503047..4504540	-	1494	WP_003086325.1	aldehyde dehydrogenase family protein	-
KCG37_RS20740 (KCG37_20740)	4504715..4505191	+	477	WP_003112531.1	hypothetical protein	-
KCG37_RS20745 (KCG37_20745)	4505194..4505469	-	276	WP_173014646.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28267.46 Da
• Isoelectric Point: 8.3428

>T199568 WP_003123189.1 NZ_CP073082:c4501129-4500374 [Pseudomonas aeruginosa]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGMRLQAPPFSDHEAALTDIA
DYRAPQKLGTAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T199568 NZ_CP097411:323452-323820 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT199568 WP_003086329.1 NZ_CP073082:c4501632-4501264 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT199568 NZ_CP097411:323234-323455 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: V6AJU7