Detailed information of TA system TA199562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5865157..5865743
• Replicon: chromosome
• Accession: NZ_CP073080
• Organism: Pseudomonas aeruginosa strain NDTH9845

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: KCG38_RS27510
• Protein ID: WP_003120987.1
• Coordinates: 5865444..5865743 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: KCG38_RS27505
• Protein ID: WP_003448662.1
• Coordinates: 5865157..5865447 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCG38_RS27490 (KCG38_27490)	5860739..5862628	+	1890	WP_211851996.1	hypothetical protein	-
KCG38_RS27495 (KCG38_27495)	5862625..5864601	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
KCG38_RS36380	5864611..5864745	+	135	WP_255253641.1	hypothetical protein	-
KCG38_RS27500 (KCG38_27500)	5864742..5865086	+	345	WP_016851612.1	hypothetical protein	-
KCG38_RS27505 (KCG38_27505)	5865157..5865447	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
KCG38_RS27510 (KCG38_27510)	5865444..5865743	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KCG38_RS27515 (KCG38_27515)	5865945..5867069	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
KCG38_RS27520 (KCG38_27520)	5867069..5868778	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
KCG38_RS27525 (KCG38_27525)	5868782..5870107	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
KCG38_RS27530 (KCG38_27530)	5870097..5870630	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5837126..5926120	88994

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T199562 WP_003120987.1 NZ_CP073080:c5865743-5865444 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T199562 NZ_CP097386:58584-59066 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT199562 WP_003448662.1 NZ_CP073080:c5865447-5865157 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT199562 NZ_CP097386:58330-58596 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: G8CP73