Detailed information of TA system TA199559

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4711523..4712781
• Replicon: chromosome
• Accession: NZ_CP073080
• Organism: Pseudomonas aeruginosa strain NDTH9845

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: KCG38_RS21950
• Protein ID: WP_003112528.1
• Coordinates: 4711523..4712278 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: KCG38_RS21955
• Protein ID: WP_003086329.1
• Coordinates: 4712413..4712781 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCG38_RS21940 (KCG38_21940)	4706696..4709239	+	2544	WP_031755663.1	acyl-homoserine-lactone acylase QuiP	-
KCG38_RS21945 (KCG38_21945)	4709394..4710755	-	1362	WP_073647918.1	DNA recombination protein RmuC	-
KCG38_RS21950 (KCG38_21950)	4711523..4712278	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
KCG38_RS21955 (KCG38_21955)	4712413..4712781	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
KCG38_RS21960 (KCG38_21960)	4712854..4714140	-	1287	WP_003163134.1	FAD-binding oxidoreductase	-
KCG38_RS21965 (KCG38_21965)	4714196..4715689	-	1494	WP_031755661.1	aldehyde dehydrogenase family protein	-
KCG38_RS21970 (KCG38_21970)	4715864..4716340	+	477	WP_003112531.1	hypothetical protein	-
KCG38_RS21975 (KCG38_21975)	4716343..4716618	-	276	WP_169057979.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T199559 WP_003112528.1 NZ_CP073080:c4712278-4711523 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T199559 NZ_CP097385:2823036-2823446 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAAATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT199559 WP_003086329.1 NZ_CP073080:c4712781-4712413 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT199559 NZ_CP097385:2822789-2823055 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7