Detailed information of TA system TA199552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 2298557..2299041
• Replicon: chromosome
• Accession: NZ_CP073075
• Organism: Gordonia polyisoprenivorans strain C

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: J8M97_RS10405
• Protein ID: WP_211544830.1
• Coordinates: 2298709..2299041 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: J8M97_RS10400
• Protein ID: WP_020171194.1
• Coordinates: 2298557..2298712 (+)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8M97_RS10370 (J8M97_10370)	2294013..2294546	+	534	WP_014362239.1	TetR/AcrR family transcriptional regulator	-
J8M97_RS10375 (J8M97_10375)	2294664..2295347	+	684	WP_020171189.1	haloacid dehalogenase type II	-
J8M97_RS10380 (J8M97_10380)	2295482..2296750	+	1269	WP_211544828.1	APC family permease	-
J8M97_RS10385 (J8M97_10385)	2296797..2297588	+	792	WP_020171191.1	ABC transporter permease	-
J8M97_RS10390 (J8M97_10390)	2297686..2297865	+	180	WP_026920097.1	hypothetical protein	-
J8M97_RS10395 (J8M97_10395)	2298005..2298346	+	342	WP_208791345.1	type II toxin-antitoxin system VapC family toxin	-
J8M97_RS10400 (J8M97_10400)	2298557..2298712	+	156	WP_020171194.1	antitoxin MazE family protein	Antitoxin
J8M97_RS10405 (J8M97_10405)	2298709..2299041	+	333	WP_211544830.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
J8M97_RS10410 (J8M97_10410)	2299261..2299626	+	366	WP_211544832.1	metalloregulator ArsR/SmtB family transcription factor	-
J8M97_RS10415 (J8M97_10415)	2299623..2300711	+	1089	WP_211544834.1	ACR3 family arsenite efflux transporter	-
J8M97_RS10420 (J8M97_10420)	2300704..2301123	+	420	WP_211544836.1	low molecular weight phosphatase family protein	-
J8M97_RS10425 (J8M97_10425)	2301142..2301624	-	483	WP_211544838.1	ArsI/CadI family heavy metal resistance metalloenzyme	-
J8M97_RS10430 (J8M97_10430)	2301727..2302092	+	366	WP_211544840.1	Rv2640c family ArsR-like transcriptional regulator	-
J8M97_RS10435 (J8M97_10435)	2302104..2303537	-	1434	WP_211545342.1	hypothetical protein	-
J8M97_RS10440 (J8M97_10440)	2303620..2303904	-	285	WP_051110333.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

• Antitoxin MazE-like (1-49)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11971.85 Da
• Isoelectric Point: 8.7143

>T199552 WP_211544830.1 NZ_CP073075:2298709-2299041 [Gordonia polyisoprenivorans]
VIRGEIWTVAGGVYASKPRPAVIVQDDLFDSTLSVVVAPITSQLIGAPLLRIRIPGDRDSISGLERDSDVMVDKLTAVRR
SNVQTRVGRLTSDQLVEVERSLMAFLGLAR

Nucleotide

• Length: 333 bp

>T199552 NZ_CP097385:684027-684245 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5650.15 Da
• Isoelectric Point: 3.7453

>AT199552 WP_020171194.1 NZ_CP073075:2298557-2298712 [Gordonia polyisoprenivorans]
MRPLQVWVPDVRTPEFAAQAHRQSVLVAEADADGDEQDFVEAVAAPWDDEA

Nucleotide

• Length: 156 bp

>AT199552 NZ_CP097385:683626-684000 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG