Detailed information of TA system TA199541

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1896928..1897704
• Replicon: chromosome
• Accession: NZ_CP073065
• Organism: Staphylococcus aureus strain 17Gst354

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: KCG41_RS09135
• Protein ID: WP_000031108.1
• Coordinates: 1896928..1897080 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: KCG41_RS09140
• Protein ID: WP_001251224.1
• Coordinates: 1897105..1897704 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCG41_RS09120 (1893133)	1893133..1893954	+	822	WP_000669380.1	RluA family pseudouridine synthase	-
KCG41_RS09125 (1894409)	1894409..1895794	-	1386	WP_000116238.1	class II fumarate hydratase	-
KCG41_RS09130 (1895990)	1895990..1896385	-	396	WP_000901018.1	hypothetical protein	-
KCG41_RS09135 (1896928)	1896928..1897080	-	153	WP_000031108.1	SAS053 family protein	Toxin
KCG41_RS09140 (1897105)	1897105..1897704	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
KCG41_RS09145 (1897863)	1897863..1898333	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
KCG41_RS09150 (1898338)	1898338..1899465	-	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
KCG41_RS09155 (1899616)	1899616..1900338	-	723	WP_031774878.1	amino acid ABC transporter ATP-binding protein	-
KCG41_RS09160 (1900331)	1900331..1901788	-	1458	WP_000649908.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T199541 WP_000031108.1 NZ_CP073065:c1897080-1896928 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T199541 NZ_CP097380:1529465-1529875 [Providencia stuartii]
GTGGTCCTGTGGAAATCTAATCTCTCCATTTCATGGAAAACTCAGCTTTTTTCAACCTGTGCTCATGGGGTAGTCGGCTT
TATTCTATTGGTCGCACCATGGGCGCCAGGGAATTCAATGGTGTGGTTGCCGCTGTTAGTGATTGTGATTGCTAGCTGGG
CAAAAAGCCAAAAAAGCATCAGCAAAATCAAAGGGACTGCGGTGCTGGTTAATGGCAATAAAGTCCAATGGAAAAAGAAT
GAGTGGAGTATCGTTAAGCAACCTTGGTGTAGCCGTGTGGGGGTATTATTGACACTCAGTGCCTTACAAGGTAAGCCACA
GAAGATCCGTTTATGGGTTGCAAAAGATGCCTTATCAGAAGAGAGCTGGCGTAATTTAAACCAACTATTACTGCAATATC
CTGATATTTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT199541 WP_001251224.1 NZ_CP073065:c1897704-1897105 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT199541 NZ_CP097380:1529218-1529484 [Providencia stuartii]
ATGGATATAGAGAATAAAGCAAGAATTCATTGGGCTTGCCGTCGTGGTATGCGTGAGTTGGATATTTCCATCATGCCATT
CTTTGAATATGAATATGAAGGACTTTCTGACAAGGACAAACGTACTTTTGTTCGTTTACTTGAGTGTGCAGACCCTGATC
TGTTTAACTGGTTAATGAATCATGGGCGCCCAGACGACGATGAGTTGTTTGGTATGGTTAAATTGATTCAGGAAAGAAAT
AAAAATCGTGGTCCTGTGGAAATCTAA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4