Detailed information of TA system TA199540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 875408..875884
• Replicon: chromosome
• Accession: NZ_CP073065
• Organism: Staphylococcus aureus strain 17Gst354

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: KCG41_RS04275
• Protein ID: WP_000052076.1
• Coordinates: 875408..875596 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: KCG41_RS04280
• Protein ID: WP_001059690.1
• Coordinates: 875624..875884 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCG41_RS04245 (871437)	871437..871919	+	483	WP_000824489.1	hypothetical protein	-
KCG41_RS04250 (872260)	872260..872946	+	687	WP_001574556.1	thermonuclease family protein	-
KCG41_RS04255 (873303)	873303..873503	+	201	WP_001059082.1	cold-shock protein	-
KCG41_RS04260 (874000)	874000..874218	-	219	WP_000005881.1	hypothetical protein	-
KCG41_RS04265 (874279)	874279..874563	-	285	WP_001004403.1	hypothetical protein	-
KCG41_RS04270 (874651)	874651..875220	-	570	WP_001106655.1	lipoprotein N-acylation protein LnsA	-
KCG41_RS04275 (875408)	875408..875596	-	189	WP_000052076.1	hypothetical protein	Toxin
KCG41_RS04280 (875624)	875624..875884	-	261	WP_001059690.1	hypothetical protein	Antitoxin
KCG41_RS04285 (876226)	876226..876429	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
KCG41_RS04290 (876426)	876426..876662	-	237	WP_000368687.1	hypothetical protein	-
KCG41_RS04295 (876887)	876887..877468	+	582	WP_001140696.1	histidine phosphatase family protein	-
KCG41_RS04300 (877541)	877541..878158	-	618	WP_000255562.1	LysE/ArgO family amino acid transporter	-
KCG41_RS04305 (878307)	878307..878801	-	495	WP_000569881.1	GNAT family N-acetyltransferase	-
KCG41_RS04310 (879200)	879200..879622	-	423	WP_000974452.1	organic hydroperoxide resistance protein	-
KCG41_RS04315 (879770)	879770..880486	+	717	WP_000150026.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T199540 WP_000052076.1 NZ_CP073065:c875596-875408 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T199540 NZ_CP097380:c878499-878296 [Providencia stuartii]
ATGACCAAAACTGATTATCTGATGCGTTTAAGAAAATGCACAACCATCGATACACTTGAGCGTGTTATTGAGAAAAATAA
GTATGAGCTTTCTGAAGATGAGTTAGAATTGTTCTATTCAGCTGCTGACCATCGCCTTGCAGAACTCACTATGAATAAAC
TTTATGACAAAATCCCTGCATCGGTTTGGAAATTTGTTAGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT199540 WP_001059690.1 NZ_CP073065:c875884-875624 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT199540 NZ_CP097380:c878947-878579 [Providencia stuartii]
ATGGATGAATATTCACCCAAAAATTATGATATATCTGAGTTAAAATACTTGTGTAACAGTTTGAATCGAGAAGCAATGTT
AAGCTTACAGAAAACTAATACACACTGGGTCAATGATTTAAGTTCACCACAAAGTGCTAGACTTAATGAACTCATTGAAC
ATATTGCAGCCTTCGTGTGGCAATTTAAGATAAAATATCCCAAAGAGAATCTCGTTATATCATTAGTTGAAGAGTATCTT
GATGAGACTTATGATTTATTCGGAAGCCCTGTAATAACTCTAAGTGAAATTATTGACTGGCAGAGCATGAATCAAAATCT
AGTTTCCGTACTTGATGATGATTTAAAGTGCCCTGCAAGCAAAACATAA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56