Detailed information of TA system TA199533

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 60234..60822
• Replicon: plasmid pDETAB13
• Accession: NZ_CP073061
• Organism: Acinetobacter baumannii strain DETAB-P39

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N9CKJ9
• Locus tag: J7D36_RS18580
• Protein ID: WP_004647749.1
• Coordinates: 60526..60822 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: J7D36_RS18575
• Protein ID: WP_006582118.1
• Coordinates: 60234..60524 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7D36_RS18550 (J7D36_18550)	56525..56806	-	282	WP_006582667.1	putative addiction module antidote protein	-
J7D36_RS18555 (J7D36_18555)	56807..57109	-	303	WP_000286963.1	type II toxin-antitoxin system RelE/ParE family toxin	-
J7D36_RS18560 (J7D36_18560)	57309..57971	+	663	WP_016653395.1	hypothetical protein	-
J7D36_RS18565 (J7D36_18565)	57979..58761	+	783	WP_006582115.1	hypothetical protein	-
J7D36_RS18570 (J7D36_18570)	59398..59820	+	423	WP_006582116.1	hypothetical protein	-
J7D36_RS18575 (J7D36_18575)	60234..60524	-	291	WP_006582118.1	putative addiction module antidote protein	Antitoxin
J7D36_RS18580 (J7D36_18580)	60526..60822	-	297	WP_004647749.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J7D36_RS18585 (J7D36_18585)	60987..61997	-	1011	WP_006582119.1	NAD(P)-dependent alcohol dehydrogenase	-
J7D36_RS18590 (J7D36_18590)	62110..63021	+	912	WP_006582120.1	LysR family transcriptional regulator	-
J7D36_RS18595 (J7D36_18595)	63566..63922	+	357	WP_000269903.1	type II toxin-antitoxin system RelE/ParE family toxin	-
J7D36_RS18600 (J7D36_18600)	63915..64217	+	303	WP_001140619.1	XRE family transcriptional regulator	-
J7D36_RS18605 (J7D36_18605)	64210..64419	+	210	WP_000069471.1	hypothetical protein	-
J7D36_RS18610 (J7D36_18610)	64544..64756	-	213	WP_016653394.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..91083	91083
	-	inside	IScluster/Tn	-	-	65515..71052	5537

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11584.25 Da
• Isoelectric Point: 5.8801

>T199533 WP_004647749.1 NZ_CP073061:c60822-60526 [Acinetobacter baumannii]
MIEIKRLPEFDEWLDGIKDNMTRIRLNRRLDKVQRGNWGDIKPLQDGVWEMREFFGSGWRMYYIQHGDVVIVMLGGGDKS
TQQQDIDRAVKLSKTLED

Nucleotide

• Length: 297 bp

>T199533 NZ_CP097377:1436728-1436979 [Actinobacillus pleuropneumoniae]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATTTAGCAGGTACAATGTCAAAAGTAACAGAAGATTTTGAACCTGT
TTTAATTACAAGAAGCAAGGGTGGAAATTGCGTACTTATGTCGTATGAGCAGTATAGTTCACTTGAAGAAACCGCCTATT
TAATGCGTTCCCCGGCTAACGCTCAAAGATTACTAGACAGCATGGCAGAATTGAAGCAAGGGAAAGGCATTGAAAGAGAG
CTAATCGAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10549.20 Da
• Isoelectric Point: 5.6088

>AT199533 WP_006582118.1 NZ_CP073061:c60524-60234 [Acinetobacter baumannii]
MVKVADLPSFDMAESLKTEEDIVMYLNMVLEDNDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRHDSAPRFDTI
NRVVNALGLKLTVQHT

Nucleotide

• Length: 291 bp

>AT199533 NZ_CP097377:1436976-1437236 [Actinobacillus pleuropneumoniae]
ATGAAGCTAACTTTCTCGTCTAATTCGTGGGAAGATTATTTATACTGGCAAAAAACCGATAAAATAATTTTGAAACGTAT
AAACAGCCTGATCAAAGATATTCAACGCCAACCATTCGAAGGCATAGGAAAACCGGAGCCGCTAAAATTCAATCTTTCGG
GATTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACAGCGTGGAAGATGAAGCAATTTTAATAGTAGCGTGT
AGATACCATTATGATCAGTAA