Detailed information of TA system TA199532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 56525..57109
• Replicon: plasmid pDETAB13
• Accession: NZ_CP073061
• Organism: Acinetobacter baumannii strain DETAB-P39

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N9JY46
• Locus tag: J7D36_RS18555
• Protein ID: WP_000286963.1
• Coordinates: 56807..57109 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: J7D36_RS18550
• Protein ID: WP_006582667.1
• Coordinates: 56525..56806 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7D36_RS18540 (J7D36_18540)	52316..54982	-	2667	WP_002055446.1	excinuclease ABC subunit UvrA	-
J7D36_RS18545 (J7D36_18545)	55025..56233	-	1209	WP_021511398.1	IS256 family transposase	-
J7D36_RS18550 (J7D36_18550)	56525..56806	-	282	WP_006582667.1	putative addiction module antidote protein	Antitoxin
J7D36_RS18555 (J7D36_18555)	56807..57109	-	303	WP_000286963.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J7D36_RS18560 (J7D36_18560)	57309..57971	+	663	WP_016653395.1	hypothetical protein	-
J7D36_RS18565 (J7D36_18565)	57979..58761	+	783	WP_006582115.1	hypothetical protein	-
J7D36_RS18570 (J7D36_18570)	59398..59820	+	423	WP_006582116.1	hypothetical protein	-
J7D36_RS18575 (J7D36_18575)	60234..60524	-	291	WP_006582118.1	putative addiction module antidote protein	-
J7D36_RS18580 (J7D36_18580)	60526..60822	-	297	WP_004647749.1	type II toxin-antitoxin system RelE/ParE family toxin	-
J7D36_RS18585 (J7D36_18585)	60987..61997	-	1011	WP_006582119.1	NAD(P)-dependent alcohol dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..91083	91083

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11545.02 Da
• Isoelectric Point: 4.6838

>T199532 WP_000286963.1 NZ_CP073061:c57109-56807 [Acinetobacter baumannii]
MYSIYTTEAFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T199532 NZ_CP097377:c723677-723288 [Actinobacillus pleuropneumoniae]
ATGTATATGCTAGATACTAATACGGTGAGTTATTTCTTTAGGCAAGATCCTACTGTGGTTAAGAAACTACAACAACTTAA
TCCGGAATTGATTTGTATTTCATCGGTCACGGCTGCAGAGTTGTTTTATGGCGTAAAAAAACGTAATAATCAGAAACTTA
CTGCATTTTTAAATACTTTTTTATCTGCGATTACGGTGATGGATTGGGATTATCAAGTGGCGGAAGTTTATGGTCAGCTG
CGAGCAGAGATGGAACGAGAAGGCAAAATCATGGGCGTTCAAGATCAAATGATTGGAGCTCATGCCCTAGCAACAGAATG
TGTTCTTGTTTCCAGCGATAAAGCCTTTCAATTTATCCCTAATCTCGTATTAGAAAATTGGTGGAAGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10139.70 Da
• Isoelectric Point: 8.0018

>AT199532 WP_006582667.1 NZ_CP073061:c56806-56525 [Acinetobacter baumannii]
MAIKLRKWDSAEHLKTEEDMQAYLQACIDESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLTI

Nucleotide

• Length: 282 bp

>AT199532 NZ_CP097377:c723938-723678 [Actinobacillus pleuropneumoniae]
ATGGAAAAGGTCGCACGTTTATTTAGAAACGGACGTAATCAAGCCGTACGATTACCGGTTGAATTTGAGTTTAATGCGAA
GCAAGTTTATGTGTTTAAAGAAGCAAACGGCGATATTACGCTTTCGACACGTTCTCGCAATCAGCAAAATTGGCAGAAAG
TCTTTGAGTTATTACCAATGCTTGAATGTGATGAACAGTTTTTATCGGAACAAGAACGCAAGCAAGAAATGGCAACTCGA
GATCCGTTCGAGGGCTTTTAA

Structures

Toxin

• AlphaFold DB: A0A836MGN2