Detailed information of TA system TA199523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2515054..2515653
• Replicon: chromosome
• Accession: NZ_CP073017
• Organism: Synechocystis sp. PCC 6803

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KBZ93_RS11735
• Protein ID: WP_010873447.1
• Coordinates: 2515054..2515431 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KBZ93_RS11740
• Protein ID: WP_010873448.1
• Coordinates: 2515432..2515653 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBZ93_RS11720 (KBZ93_11725)	2510418..2511113	-	696	WP_010873444.1	aldehyde oxygenase (deformylating)	-
KBZ93_RS11725 (KBZ93_11730)	2511371..2512468	-	1098	WP_014407137.1	glucose-1-phosphate thymidylyltransferase	-
KBZ93_RS11730 (KBZ93_11735)	2512710..2514878	+	2169	WP_010873446.1	glycine--tRNA ligase subunit beta	-
KBZ93_RS11735 (KBZ93_11740)	2515054..2515431	-	378	WP_010873447.1	type II toxin-antitoxin system VapC family toxin	Toxin
KBZ93_RS11740 (KBZ93_11745)	2515432..2515653	-	222	WP_010873448.1	DUF2281 domain-containing protein	Antitoxin
KBZ93_RS11745 (KBZ93_11750)	2515718..2517088	-	1371	WP_010873449.1	FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (2-124)

Antitoxin

• Domain of unknown function DUF2281 (6-72)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14761.18 Da
• Isoelectric Point: 7.0607

>T199523 WP_010873447.1 NZ_CP073017:c2515431-2515054 [Synechocystis sp. PCC 6803]
MLLDTHTLLWFLDNDIKLPSHLKSLIEAKPKVYVSIVSLWEIAIKLNINKLRLNYAFTDLENLLADLNISVLNITWQHLE
ACRNLPLHHRDPFDRMIIAQAQQHNFSVISKDENFKKYNVNLIWE

Nucleotide

• Length: 378 bp

>T199523 NZ_CP097370:c3925227-3924913 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8355.66 Da
• Isoelectric Point: 4.3855

>AT199523 WP_010873448.1 NZ_CP073017:c2515653-2515432 [Synechocystis sp. PCC 6803]
MTDTALLEKINALPDAMKIEVEHFVEFLLTKQPPVSPVTNDGQKKYRQAGVLKGKIWMADDFDAPLEEMQEYM

Nucleotide

• Length: 222 bp

>AT199523 NZ_CP097370:c3925463-3925230 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG