Detailed information of TA system TA199517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 539264..539805
• Replicon: chromosome
• Accession: NZ_CP073015
• Organism: Flavobacterium sp. CS20

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: IGB25_RS02675
• Protein ID: WP_211066052.1
• Coordinates: 539503..539805 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: IGB25_RS02670
• Protein ID: WP_211066051.1
• Coordinates: 539264..539506 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGB25_RS02640 (IGB25_02640)	534366..535652	-	1287	WP_211066046.1	DUF2851 family protein	-
IGB25_RS02645 (IGB25_02645)	535652..536332	-	681	WP_247653580.1	SGNH/GDSL hydrolase family protein	-
IGB25_RS02650 (IGB25_02650)	536322..537182	-	861	WP_211066047.1	alpha/beta hydrolase	-
IGB25_RS02655 (IGB25_02655)	537562..537879	-	318	WP_211066048.1	hypothetical protein	-
IGB25_RS02660 (IGB25_02660)	537883..538029	-	147	WP_211066049.1	hypothetical protein	-
IGB25_RS02665 (IGB25_02665)	538206..539171	+	966	WP_211066050.1	NAD-dependent epimerase/dehydratase family protein	-
IGB25_RS02670 (IGB25_02670)	539264..539506	+	243	WP_211066051.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
IGB25_RS02675 (IGB25_02675)	539503..539805	+	303	WP_211066052.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IGB25_RS02680 (IGB25_02680)	539883..540902	+	1020	WP_211066053.1	Holliday junction branch migration DNA helicase RuvB	-
IGB25_RS02685 (IGB25_02685)	540914..541546	+	633	WP_211066054.1	Crp/Fnr family transcriptional regulator	-
IGB25_RS02690 (IGB25_02690)	541543..542007	+	465	WP_211066055.1	isoprenylcysteine carboxylmethyltransferase family protein	-
IGB25_RS02695 (IGB25_02695)	542352..543749	+	1398	WP_211066824.1	arginine decarboxylase	-
IGB25_RS02700 (IGB25_02700)	543742..544616	+	875	Protein_531	agmatinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-94)

Antitoxin

• Antitoxin ParD (4-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 12225.20 Da
• Isoelectric Point: 10.3171

>T199517 WP_211066052.1 NZ_CP073015:539503-539805 [Flavobacterium sp. CS20]
MSDKKYRISQQAIDDLNDIWIYIYHKWSKEQADRYYALIIEEIKFIAQNYFTGKSIDKTRKNYRVTKVKSHLIFYRKVDN
GLVEVVRILHQRMNIKNRLP

Nucleotide

• Length: 303 bp

>T199517 NZ_CP097370:c1957985-1957611 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTACTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9255.51 Da
• Isoelectric Point: 5.1905

>AT199517 WP_211066051.1 NZ_CP073015:539264-539506 [Flavobacterium sp. CS20]
MGKNTSVSLGNHFESFIQEELKTGKYSSVSEVIRSALRLLEREEIKEKELIKALKIGEESGFVENFDFEQNLLDLHRQKL

Nucleotide

• Length: 243 bp

>AT199517 NZ_CP097370:c1958442-1958074 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCAGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA