Detailed information of TA system TA199498

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 2459150..2459926
• Replicon: chromosome
• Accession: NZ_CP073012
• Organism: Staphylococcus aureus strain SA14+

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: KAJ74_RS12060
• Protein ID: WP_000031108.1
• Coordinates: 2459774..2459926 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: -
• Locus tag: KAJ74_RS12055
• Protein ID: WP_001251230.1
• Coordinates: 2459150..2459749 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KAJ74_RS12035 (KAJ74_12035)	2455066..2456523	+	1458	WP_000649908.1	ABC transporter permease subunit	-
KAJ74_RS12040 (KAJ74_12040)	2456516..2457238	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
KAJ74_RS12045 (KAJ74_12045)	2457389..2458516	+	1128	WP_031590038.1	tRNA epoxyqueuosine(34) reductase QueG	-
KAJ74_RS12050 (KAJ74_12050)	2458521..2458991	+	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
KAJ74_RS12055 (KAJ74_12055)	2459150..2459749	+	600	WP_001251230.1	hypothetical protein	Antitoxin
KAJ74_RS12060 (KAJ74_12060)	2459774..2459926	+	153	WP_000031108.1	SAS053 family protein	Toxin
KAJ74_RS12065 (KAJ74_12065)	2460762..2461157	+	396	WP_000901023.1	hypothetical protein	-
KAJ74_RS12070 (KAJ74_12070)	2461353..2462738	+	1386	WP_000116229.1	class II fumarate hydratase	-
KAJ74_RS12075 (KAJ74_12075)	2463190..2464011	-	822	WP_000669376.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T199498 WP_000031108.1 NZ_CP073012:2459774-2459926 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T199498 NZ_CP097367:3564569-3564787 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22369.51 Da
• Isoelectric Point: 4.9728

>AT199498 WP_001251230.1 NZ_CP073012:2459150-2459749 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKYAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT199498 NZ_CP097367:3564169-3564543 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: X5E2E6