Detailed information of TA system TA199486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 4049274..4049922
• Replicon: chromosome
• Accession: NZ_CP073011
• Organism: Virgibacillus pantothenticus strain DSM 26

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0L0QSJ8
• Locus tag: KBP50_RS18880
• Protein ID: WP_050351134.1
• Coordinates: 4049274..4049633 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: KBP50_RS18885
• Protein ID: WP_077298901.1
• Coordinates: 4049638..4049922 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBP50_RS18855 (KBP50_18855)	4044510..4044845	-	336	WP_050351140.1	STAS domain-containing protein	-
KBP50_RS18860 (KBP50_18860)	4045197..4046210	-	1014	WP_050351139.1	PP2C family protein-serine/threonine phosphatase	-
KBP50_RS18865 (KBP50_18865)	4046392..4046793	-	402	WP_050351138.1	anti-sigma regulatory factor	-
KBP50_RS18870 (KBP50_18870)	4046797..4047153	-	357	WP_050351137.1	STAS domain-containing protein	-
KBP50_RS18875 (KBP50_18875)	4047159..4048028	-	870	WP_050351136.1	RsbT co-antagonist protein RsbRA	-
KBP50_RS22275	4048945..4049127	+	183	WP_050351135.1	hypothetical protein	-
KBP50_RS18880 (KBP50_18880)	4049274..4049633	-	360	WP_050351134.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KBP50_RS18885 (KBP50_18885)	4049638..4049922	-	285	WP_077298901.1	antitoxin	Antitoxin
KBP50_RS18890 (KBP50_18890)	4050321..4051439	-	1119	WP_244969436.1	alanine racemase	-
KBP50_RS18895 (KBP50_18895)	4052039..4053061	-	1023	WP_050351132.1	outer membrane lipoprotein carrier protein LolA	-
KBP50_RS18900 (KBP50_18900)	4053227..4054747	-	1521	WP_050351131.1	bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13455.69 Da
• Isoelectric Point: 8.4535

>T199486 WP_050351134.1 NZ_CP073011:c4049633-4049274 [Virgibacillus pantothenticus]
MIVQRGEVYFADLSPVVGSEQGGIRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFDRDSVILLEQI
RTLDKQRLTDKITKLDKEMMKKINQALEISLGLKDMYDQ

Nucleotide

• Length: 360 bp

>T199486 NZ_CP097367:291052-291579 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10867.21 Da
• Isoelectric Point: 4.5792

>AT199486 WP_077298901.1 NZ_CP073011:c4049922-4049638 [Virgibacillus pantothenticus]
MSESLQEVLVKIPKNLLNEVDGLVKYENSDLSDFICQATRNYLQHKRDEHFQQFRETMEKGYAEMGRINLTIASEAFQAE
EEAKNTLERSVIGV

Nucleotide

• Length: 285 bp

>AT199486 NZ_CP097367:290779-291045 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: A0A0L0QSJ8