Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5370641..5371266 Replicon chromosome
Accession NZ_CP073002
Organism Klebsiella pneumoniae strain FO15

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KBT91_RS26460 Protein ID WP_002882817.1
Coordinates 5370641..5371024 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KBT91_RS26465 Protein ID WP_004150355.1
Coordinates 5371024..5371266 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBT91_RS26445 5368007..5368909 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KBT91_RS26450 5368906..5369541 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KBT91_RS26455 5369538..5370467 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KBT91_RS26460 5370641..5371024 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KBT91_RS26465 5371024..5371266 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KBT91_RS26470 5371471..5372388 + 918 WP_002882812.1 alpha/beta hydrolase -
KBT91_RS26475 5372402..5373343 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KBT91_RS26480 5373388..5373825 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KBT91_RS26485 5373822..5374682 - 861 WP_002882807.1 virulence factor BrkB family protein -
KBT91_RS26490 5374676..5375275 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T199476 WP_002882817.1 NZ_CP073002:c5371024-5370641 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T199476 NZ_CP097360:3619442-3619660 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT199476 WP_004150355.1 NZ_CP073002:c5371266-5371024 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT199476 NZ_CP097360:3619042-3619416 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References